Ho, Samuel and Tirrell, David Anthony (2019) Enzymatic labeling of bacterial proteins for live-cell super-resolution imaging. In: 257th ACS National Meeting & Exposition, 31 March - 4 April 2019, Orlando, FL. https://resolver.caltech.edu/CaltechAUTHORS:20190325-084657712
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Abstract
Mol. probes that illuminate the subcellular organization of prokaryotic organisms are essential for our understanding of the biol. world. We describe here a strategy for labeling bacterial proteins in situ with azido fatty acids using the enzyme N-myristoyltransferase. Cell-permeant photoswitchable fluorescent dyes are conjugated to target proteins through means of strain-promoted azide-alkyne cycloaddn. to enable super-resoln. imaging in live Escherichia coli cells. We demonstrate the method by labeling and imaging bacterial chemotaxis proteins and cell division proteins. The method provides a general framework for live-cell protein imaging with nanometer resoln.
Item Type: | Conference or Workshop Item (Paper) | ||||||
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Additional Information: | © 2019 American Chemical Society. | ||||||
Record Number: | CaltechAUTHORS:20190325-084657712 | ||||||
Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20190325-084657712 | ||||||
Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | ||||||
ID Code: | 94092 | ||||||
Collection: | CaltechAUTHORS | ||||||
Deposited By: | Tony Diaz | ||||||
Deposited On: | 25 Mar 2019 15:50 | ||||||
Last Modified: | 03 Oct 2019 21:00 |
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