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Enzymatic labeling of bacterial proteins for live-cell super-resolution imaging

Ho, Samuel and Tirrell, David Anthony (2019) Enzymatic labeling of bacterial proteins for live-cell super-resolution imaging. In: 257th ACS National Meeting & Exposition, 31 March - 4 April 2019, Orlando, FL.

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Mol. probes that illuminate the subcellular organization of prokaryotic organisms are essential for our understanding of the biol. world. We describe here a strategy for labeling bacterial proteins in situ with azido fatty acids using the enzyme N-myristoyltransferase. Cell-permeant photoswitchable fluorescent dyes are conjugated to target proteins through means of strain-promoted azide-alkyne cycloaddn. to enable super-resoln. imaging in live Escherichia coli cells. We demonstrate the method by labeling and imaging bacterial chemotaxis proteins and cell division proteins. The method provides a general framework for live-cell protein imaging with nanometer resoln.

Item Type:Conference or Workshop Item (Paper)
Related URLs:
URLURL TypeDescription Website
Ho, Samuel0000-0001-7647-0752
Tirrell, David Anthony0000-0003-3175-4596
Additional Information:© 2019 American Chemical Society.
Record Number:CaltechAUTHORS:20190325-084657712
Persistent URL:
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:94092
Deposited By: Tony Diaz
Deposited On:25 Mar 2019 15:50
Last Modified:03 Oct 2019 21:00

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