Innovation by evolution: Bringing new chemistry to life

Abstract

Not satisfied with nature's vast catalyst repertoire, we want to create new protein catalysts and expand the space of genetically encoded enzyme functions. I will describe how we can use the most powerful biol. design process, evolution, to optimize existing enzymes and invent new ones, thereby circumventing our profound ignorance of how sequence encodes function. Using mechanistic understanding and mimicking nature's evolutionary processes, we can generate whole new enzyme families that catalyze synthetically important reactions not known in biol. Recent successes include selective carbene insertion to form C-Si and C-B bonds, and alkyne cyclopropanation to make highly strained carbocycles, all in living cells. Extending the capabilities and uncovering the mechanisms of these new enzymes derived from natural iron-heme proteins provides a basis for discovering new biocatalysts for increasingly challenging reactions. These new capabilities increase the scope of mols. and materials we can build using synthetic biol. and move us closer to a sustainable world where chem. synthesis can be fully programmed in DNA.