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Countercurrent separation: A new method for studying behavior of small aquatic organisms

Dusenbery, David B. (1973) Countercurrent separation: A new method for studying behavior of small aquatic organisms. Proceedings of the National Academy of Sciences of the United States of America, 70 (5). pp. 1349-1352. ISSN 0027-8424. PMCID PMC433494. doi:10.1073/pnas.70.5.1349.

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A new method for the analysis of behavior in small free-swimming aquatic organisms is described. In this procedure, called countercurrent separation, a dense solution flows down along the bottom of an inclined chamber while a light solution flows in the opposite direction, upward along the top of the chamber. The attraction of animals (injected into the center of the chamber) to one solution or the other is then determined by observing the proportion of animals that emerges from the chamber in that solution. When used with the nematode Caenorhabditis elegans, it is estimated that the apparatus is equivalent to at least nine theoretical plates.

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Additional Information:© 1973 by the National Academy of Sciences. Communicated by Ray D. Owen, March 5, 1973. This work was largely supported by National Institutes of Health Fellowship 5 FO2 GM 45091 from the Institute of General Medical Sciences. I thank Dr. Ray D. Owen [and his AEC contract AT(04-3)767], and Dr. Richard L. Russell (and his USPHS Grant NS 09654-02 and Sloan Foundation Grant in Neuroscience) for providing both material and intangible aid to this work. In addition, I thank Dr. Sydney Brenner for demonstrating the advantages of C. elegans for genetic studies, Dr. Ruth Pertel for the information that this nematode is attracted to salts, and Dr. Samuel Ward for communicating the results of his studies on chemotaxis in C. elegans prior to publication.
Funding AgencyGrant Number
NIH Postdoctoral Fellowship5 FO2 GM 45091
NIHNS 09654-02
Alfred P. Sloan FoundationUNSPECIFIED
Subject Keywords:nematode; Caenorhabditis elegans; chemotaxis
Issue or Number:5
PubMed Central ID:PMC433494
Record Number:CaltechAUTHORS:DUSpnas73
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:9412
Deposited By: Tony Diaz
Deposited On:20 Dec 2007
Last Modified:13 Jul 2021 16:08

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