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Addressable, "Packet-Based" Intercellular Communication through Plasmid Conjugation

Marken, John P. and Murray, Richard M. (2019) Addressable, "Packet-Based" Intercellular Communication through Plasmid Conjugation. . (Unpublished)

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We develop a system for implementing "packet-based" intercellular communication in an engineered bacterial population via conjugation. Our system uses gRNA-based identification markers that allow messages to be addressed to specific strains via Cas9-mediated cleavage of messages sent to the wrong recipient, which we show reduces plasmid transfer by four orders of magnitude. Integrase-mediated editing of the address on the message plasmid allows cells to dynamically update the message's recipients in vivo. As a proof-of-concept demonstration of our system, we propose a linear path scheme that would propagate a message sequentially through the strains of a population in a defined order.

Item Type:Report or Paper (Discussion Paper)
Related URLs:
URLURL TypeDescription Paper
Marken, John P.0000-0001-9696-088X
Murray, Richard M.0000-0002-5785-7481
Additional Information:The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-ND 4.0 International license. We thank Andrey Shur and Andy Halleran for insightful discussions. The F_HR plasmid used in [9] was generously provided by Tatiana Dimitriu. PSal, PLas, and PCin promoters, as well as their respective activators NahR, LasR, and CinR, were a generous gift from Adam Meyer (now documented in [23]). Plasmid vectors were provided by Douglas Densmore at the Crossdisciplinary Integration of Design Automation Research lab (Addgene Kit # 1000000059). This research is supported by the Institute for Collaborative Biotechnologies through cooperative agreement W911NF-19-2-0026 and grant W911NF-09-0001 from the U.S. Army Research Office. The content of the information on this page does not necessarily reflect the position or the policy of the Government, and no official endorsement should be inferred.
Funding AgencyGrant Number
Army Research Office (ARO)W911NF-19-2-0026
Army Research Office (ARO)W911NF-09-0001
Record Number:CaltechAUTHORS:20190328-093304845
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Official Citation:Addressable, "Packet-Based" Intercellular Communication through Plasmid Conjugation John P Marken, Richard M Murray bioRxiv 591552; doi:
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:94234
Deposited By: George Porter
Deposited On:28 Mar 2019 22:12
Last Modified:16 Nov 2021 17:03

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