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Cytochrome b562 folding triggered by electron transfer: Approaching the speed limit for formation of a four-helix-bundle protein

Wittung-Stafshede, Pernilla and Lee, Jennifer C. and Winkler, Jay R. and Gray, Harry B. (1999) Cytochrome b562 folding triggered by electron transfer: Approaching the speed limit for formation of a four-helix-bundle protein. Proceedings of the National Academy of Sciences of the United States of America, 96 (12). pp. 6587-6590. ISSN 0027-8424. PMCID PMC21958.

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Ferrocytochrome b(562) [Fe(II)cyt b(562)] folding can be triggered by photoinduced electron transfer to unfolded Fe (III)cyt b(562) in 2-3 M guanidine hydrochloride solutions. The folding rates increase with decreasing guanidine hydrochloride; the extrapolated time constant for this folding process in the absence of denaturant (5 mu s) is near the predicted value for intrachain diffusion. The relatively smooth energy landscape indicated for Fe(II)cyt b(562) folding accords with the helical, highly symmetrical structure of the protein.

Item Type:Article
Related URLs:
URLURL TypeDescription CentralArticle
Wittung-Stafshede, Pernilla0000-0003-1058-1964
Winkler, Jay R.0000-0002-4453-9716
Gray, Harry B.0000-0002-7937-7876
Additional Information:© 1999 by the National Academy of Sciences. Contributed by Harry B. Gray, April 6, 1999. We thank Kevin Plaxco and Peter Wolynes for communication of unpublished results as well as several stimulating discussions. P.W.-S. acknowledges a postdoctoral fellowship from the Swedish Technical Research Council. J.C.L. acknowledges a graduate fellowship from the Ralph M. Parsons Foundation. This work was supported by the National Science Foundation (Grant MCB 9630465). The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.
Funding AgencyGrant Number
Swedish Technical Research CouncilUNSPECIFIED
Ralph M. Parsons FoundationUNSPECIFIED
Subject Keywords:protein folding, guanidine hydrochloride, contact order, A binding-protein, 4-helix bundle, Escherichia-coli, kinetics, thermodynamics, perspective, mechanism, dynamics, events
Issue or Number:12
PubMed Central ID:PMC21958
Record Number:CaltechAUTHORS:WITpnas99
Persistent URL:
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:943
Deposited By: Tony Diaz
Deposited On:15 Nov 2005
Last Modified:02 Oct 2019 22:38

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