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Determinants for the Subcellular Localization and Function of a Nonessential SEDS Protein

Real, Gonçalo and Fay, Allison and Eldar, Avigdor and Pinto, Sérgio M. and Henriques, Adriano O. and Dworkin, Jonathan (2008) Determinants for the Subcellular Localization and Function of a Nonessential SEDS Protein. Journal of Bacteriology, 190 (1). pp. 363-376. ISSN 0021-9193. https://resolver.caltech.edu/CaltechAUTHORS:REAjbact08

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Abstract

The Bacillus subtilis SpoVE integral membrane protein is essential for the heat resistance of spores, probably because of its involvement in spore peptidoglycan synthesis. We found that an SpoVE-yellow fluorescent protein (YFP) fusion protein becomes localized to the forespore during the earliest stages of engulfment, and this pattern is maintained throughout sporulation. SpoVE belongs to a well-conserved family of proteins that includes the FtsW and RodA proteins of B. subtilis. These proteins are involved in bacterial shape determination, although their function is not known. FtsW is necessary for the formation of the asymmetric septum in sporulation, and we found that an FtsW-YFP fusion localized to this structure prior to the initiation of engulfment in a nonoverlapping pattern with SpoVE-cyan fluorescent protein. Since FtsW and RodA are essential for normal growth, it has not been possible to identify loss-of-function mutations that would greatly facilitate analysis of their function. We took advantage of the fact that SpoVE is not required for growth to obtain point mutations in SpoVE that block the development of spore heat resistance but that allow normal protein expression and targeting to the forespore. These mutant proteins will be invaluable tools for future experiments aimed at elucidating the function of members of the SEDS ("shape, elongation, division, and sporulation") family of proteins.


Item Type:Article
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https://doi.org/10.1128/JB.01482-07DOIUNSPECIFIED
Additional Information:Copyright © 2008, American Society for Microbiology. Received 13 September 2007/ Accepted 21 October 2007. Published ahead of print on 2 November 2007. We thank Colin Manoil for the gift of strains, Teresa Costa for the construction of pTC30, and Anabela Isidro for the anti-GFP antibody. This work was funded by grants POCTI/BCI/48647/2002 and POCI/BIA-BCM/60855/2004 from the Fundação para a Ciência e a Tecnologia (F.C.T.) to A.O.H. and by an Irma T. Hirschl scholar award and startup funds from the Department of Microbiology, Columbia University, to J.D. G.R. was the recipient of a postdoctoral fellowship (SFRH/BPD/20668/2004) from the F.C.T. A.F. was supported by NIH training grant AI007161-29. These authors [G.R. and A.F.] contributed equally to the work. Supplemental material for this article may be found at http://jb.asm.org/.
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Deposited On:03 Jan 2008
Last Modified:03 Oct 2019 00:00

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