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Quantitative cell nuclear imaging by dual-view optical-resolution photoacoustic microscopy

Wong, Terence T. W. and Cai, De and Zhu, Liren and Shi, Junhui and Chen, Sung-Liang and Wang, Lihong V. (2019) Quantitative cell nuclear imaging by dual-view optical-resolution photoacoustic microscopy. In: Photons Plus Ultrasound: Imaging and Sensing 2019. Proceedings of SPIE. No.10878. Society of Photo-optical Instrumentation Engineers (SPIE) , Bellingham, WA, Art. No. 108780R. ISBN 9781510623989. http://resolver.caltech.edu/CaltechAUTHORS:20190404-110929115

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Abstract

An estimated ~250,000 new cases of both invasive and non-invasive breast cancer were diagnosed in US women almost every year. To reduce the local recurrence rate, the breast conserving surgery (BCS) is widely used as the initial therapy, which is to excise the tumor with a rim of normal surrounding tissue such that no cancer cells remain at the cut margin, Patients with positive margin commonly require a second surgical procedure to obtain clear margins. To this end, optical-resolution photoacoustic microscopy (OR-PAM) with ultraviolet (UV) laser illumination (OR-UV-PAM) has been developed for providing label-free, high-resolution, and histology like imaging of fixed, unprocessed breast tissue. To further improve the performance of OR-UV-PAM, here, we introduce dual-view UV-PAM (DV-UV-PAM) to significantly improve the axial resolution, achieving three-dimensional (3D) resolution isotropy. We first use 0.5 μm polystyrene beads and carbon fibers to validate the resolution isotropy improvement. Imaging of mouse brain slices further demonstrates the improved resolution isotropy, revealing the 3D structure of cell nuclei in detail, which facilitates quantitative cell nuclear analysis.


Item Type:Book Section
Related URLs:
URLURL TypeDescription
https://doi.org/10.1117/12.2509219DOIArticle
ORCID:
AuthorORCID
Wong, Terence T. W.0000-0001-6399-758X
Zhu, Liren0000-0002-6338-9338
Shi, Junhui0000-0002-5741-2781
Chen, Sung-Liang0000-0002-0572-5110
Wang, Lihong V.0000-0001-9783-4383
Additional Information:© 2019 Society of Photo-optical Instrumentation Engineers (SPIE).
Subject Keywords:Photoacoustic microscopy, photoacoustic tomography, cell nuclear imaging, label-free imaging, quantitative imaging
Record Number:CaltechAUTHORS:20190404-110929115
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20190404-110929115
Official Citation:Terence T. W. Wong, De Cai, Liren Zhu, Junhui Shi, Sung-Liang Chen, and Lihong V. Wang "Quantitative cell nuclear imaging by dual-view optical-resolution photoacoustic microscopy", Proc. SPIE 10878, Photons Plus Ultrasound: Imaging and Sensing 2019, 108780R (27 February 2019); doi: 10.1117/12.2509219; https://doi.org/10.1117/12.2509219
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:94457
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:04 Apr 2019 18:19
Last Modified:04 Apr 2019 18:19

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