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High-resolution high-contrast mid-infrared imaging of fresh biological samples with ultraviolet-localized photoacoustic microscopy (Conference Presentation)

Shi, Junhui and Wong, Terence T. W. and He, Yun and Li, Lei and Zhang, Ruiying and Hwang, Jeeseong and Maslov, Konstantin and Wang, Lihong (2019) High-resolution high-contrast mid-infrared imaging of fresh biological samples with ultraviolet-localized photoacoustic microscopy (Conference Presentation). In: Photons Plus Ultrasound: Imaging and Sensing 2019. Proceedings of SPIE. No.10878. Society of Photo-optical Instrumentation Engineers (SPIE) , Bellingham, WA, Art. No. 1087825. ISBN 9781510623989. http://resolver.caltech.edu/CaltechAUTHORS:20190404-143835667

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Abstract

Label-free mid-infrared (MIR) imaging provides rich chemical and structural information of biological tissues without staining. Conventionally, the long MIR wavelength severely limits the lateral resolution owing to optical diffraction; moreover, the strong MIR absorption of water ubiquitous in fresh biological samples results in high background and low contrast. Here, we present a novel approach, called ultraviolet-localized MIR photoacoustic microscopy (ULM-PAM), to achieve high-resolution and water-background–free MIR imaging of fresh biological samples. In our approach, a pulsed MIR laser thermally excites the sample at the focal spot, and a pulsed ultraviolet (UV) laser photoacoustically detects the resulting transient temperature rise owing to the Grüneisen relaxation effect, thereby reporting the intensity of the MIR absorption by the sample. The imaging resolution of our method is determined by the wavelength of the UV laser, which is one order of magnitude shorter than that of the mid-IR laser (2.5 μm to 12 μm). In addition, in the UV region from 200 nm to 230 nm, most important organic molecules in biological tissues, including proteins, lipids and nuclei acids, have strong absorption, while water is transparent. Therefore, our method can achieve high resolution and water-background free IR imaging of fresh biological samples. For cell cultures, our method achieved high-resolution and high-contrast infrared imaging of lipids, proteins. The capability of label-free histology of this method is also demonstrated in thick biological tissues, such as brain slices. Our approach provides convenient high-resolution and high-contrast MIR imaging, which can benefit diagnosis of fresh biological samples.


Item Type:Book Section
Related URLs:
URLURL TypeDescription
https://doi.org/10.1117/12.2515616DOIArticle
ORCID:
AuthorORCID
Shi, Junhui0000-0002-5741-2781
Wong, Terence T. W.0000-0001-6399-758X
Zhang, Ruiying0000-0002-0092-0814
Wang, Lihong0000-0001-9783-4383
Additional Information:© 2019 Society of Photo-optical Instrumentation Engineers (SPIE).
Record Number:CaltechAUTHORS:20190404-143835667
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20190404-143835667
Official Citation:Junhui Shi, Terence T. W. Wong, Yun He, Lei Li, Ruiying Zhang, Jeeseong Hwang, Konstantin Maslov, and Lihong Wang "High-resolution high-contrast mid-infrared imaging of fresh biological samples with ultraviolet-localized photoacoustic microscopy (Conference Presentation)", Proc. SPIE 10878, Photons Plus Ultrasound: Imaging and Sensing 2019, 1087825 (4 March 2019); doi: 10.1117/12.2515616; https://doi.org/10.1117/12.2515616
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:94470
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:04 Apr 2019 21:48
Last Modified:04 Apr 2019 21:48

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