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Dual-wavelength high-speed functional photoacoustic microscopy of mouse brain with a Raman laser at 1-MHz A-line rate (Conference Presentation)

He, Yun and Shi, Junhui and Maslov, Konstantin I. and Wang, Lihong V. (2019) Dual-wavelength high-speed functional photoacoustic microscopy of mouse brain with a Raman laser at 1-MHz A-line rate (Conference Presentation). In: Photons Plus Ultrasound: Imaging and Sensing 2019. Proceedings of SPIE. No.10878. Society of Photo-optical Instrumentation Engineers (SPIE) , Bellingham, WA, Art. No. 1087824. ISBN 9781510623989. https://resolver.caltech.edu/CaltechAUTHORS:20190404-150606290

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Abstract

Label-free functional photoacoustic microscopy (fPAM) has become a popular technology in small-animal hemodynamic studies. Here we report a stimulated-Raman-scattering-based (SRS) dual-wavelength high-speed fPAM that has achieved volumetric imaging at a 1 MHz A-line rate with capillary-level resolution. Potassium gadolinium tungstate (KGd(WO4)2) crystal is used as a Raman shifter to convert the pump 532 nm picosecond-pulsed laser to the first order Stokes line at 558 nm through the SRS effect with ~40% efficiency and a much narrower line width compared with previous fiber-based SRS PAMs. We also developed a water-immersible micro-electro-mechanical system scanner for scanning a ~4-mm range at a 500 Hz B-scan rate, while maintaining the optic-acoustic confocal alignment. This scanner is assembled entirely from commercially available components, facilitating replication. The detection sensitivity of our fPAM is also improved by employing a high numerical aperture polyvinylidene fluoride ultrasonic transducer, whose acoustic impedance matches better with tissue coupling medium than traditional ceramic transducers. The high sensitivity combined with ~2.4 µm resolution enabled our fPAM to image single red blood cells with a signal-to-noise ratio of ~27 dB. Compared with our previous laser-pulse-width based fPAM, we achieved simultaneous imaging of hemoglobin concentration and oxygenation with a 5-fold increase in imaging speed. Moreover, our system works in a convenient free-space manner compared to previous SRS-based PAMs. We applied it to imaging vasculature and blood oxygen saturation on mouse brains in both resting and stimulated states.


Item Type:Book Section
Related URLs:
URLURL TypeDescription
https://doi.org/10.1117/12.2510658DOIArticle
ORCID:
AuthorORCID
Shi, Junhui0000-0002-5741-2781
Wang, Lihong V.0000-0001-9783-4383
Additional Information:© 2019 Society of Photo-optical Instrumentation Engineers (SPIE).
Series Name:Proceedings of SPIE
Issue or Number:10878
Record Number:CaltechAUTHORS:20190404-150606290
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20190404-150606290
Official Citation:Yun He, Junhui Shi, Konstantin I. Maslov, and Lihong V. Wang "Dual-wavelength high-speed functional photoacoustic microscopy of mouse brain with a Raman laser at 1-MHz A-line rate (Conference Presentation)", Proc. SPIE 10878, Photons Plus Ultrasound: Imaging and Sensing 2019, 1087824 (4 March 2019); doi: 10.1117/12.2510658; https://doi.org/10.1117/12.2510658
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:94473
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:04 Apr 2019 23:46
Last Modified:03 Oct 2019 21:04

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