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Harmonically matched grating-based full-field quantitative high-resolution phase microscope for observing dynamics of transparent biological samples

Wu, Jigang and Yaqoob, Zahid and Heng, Xin and Yang, Changhuei (2007) Harmonically matched grating-based full-field quantitative high-resolution phase microscope for observing dynamics of transparent biological samples. Optics Express, 15 (26). pp. 18141-18155. ISSN 1094-4087. https://resolver.caltech.edu/CaltechAUTHORS:WUJoe07.pdf

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Abstract

We have developed a full-field high resolution quantitative phase imaging technique for observing dynamics of transparent biological samples. By using a harmonically matched diffraction grating pair (600 and 1200 lines/mm), we were able to obtain non-trivial phase difference (other than 0° or 180°) between the output ports of the gratings. Improving upon our previous design, our current system mitigates astigmatism artifacts and is capable of high resolution imaging. This system also employs an improved phase extraction algorithm. The system has a lateral resolution of 1.6 μm and a phase sensitivity of 62 mrad. We employed the system to acquire high resolution phase images of onion skin cells and a phase movie of amoeba proteus in motion.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://www.opticsinfobase.org/abstract.cfm?URI=oe-15-26-18141OtherUNSPECIFIED
ORCID:
AuthorORCID
Yang, Changhuei0000-0001-8791-0354
Additional Information:© 2007 Optical Society of America. Original Manuscript: September 6, 2007. Manuscript Accepted: December 14, 2007. Revised Manuscript: December 4, 2007. Published: December 19, 2007. This work is supported by NSF career award BES-0547657.
Funders:
Funding AgencyGrant Number
NSFBES-0547657
Subject Keywords:Phase measurement; Diffraction gratings; Medical and biological imaging
Issue or Number:26
Record Number:CaltechAUTHORS:WUJoe07.pdf
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:WUJoe07.pdf
Official Citation:J. Wu, Z. Yaqoob, X. Heng, X. Cui, and C. Yang, "Harmonically matched grating-based full-field quantitative high-resolution phase microscope for observing dynamics of transparent biological samples," Opt. Express 15, 18141-18155 (2007) http://www.opticsinfobase.org/abstract.cfm?URI=oe-15-26-18141
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:9452
Collection:CaltechAUTHORS
Deposited By: Kristin Buxton
Deposited On:07 Jan 2008
Last Modified:09 Mar 2020 13:19

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