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Actomyosin polarisation through PLC-PKC triggers symmetry breaking of the mouse embryo

Zhu, Meng and Leung, Chuen Yan and Shahbazi, Marta N. and Zernicka-Goetz, Magdalena (2017) Actomyosin polarisation through PLC-PKC triggers symmetry breaking of the mouse embryo. Nature Communications, 8 . Art. No. 921. ISSN 2041-1723. PMCID PMC5640629. https://resolver.caltech.edu/CaltechAUTHORS:20190405-170314469

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Abstract

Establishment of cell polarity in the mammalian embryo is fundamental for the first cell fate decision that sets aside progenitor cells for both the new organism and the placenta. Yet the sequence of events and molecular mechanism that trigger this process remain unknown. Here, we show that de novo polarisation of the mouse embryo occurs in two distinct phases at the 8-cell stage. In the first phase, an apical actomyosin network is formed. This is a pre-requisite for the second phase, in which the Par complex localises to the apical domain, excluding actomyosin and forming a mature apical cap. Using a variety of approaches, we also show that phospholipase C-mediated PIP_2 hydrolysis is necessary and sufficient to trigger the polarisation of actomyosin through the Rho-mediated recruitment of myosin II to the apical cortex. Together, these results reveal the molecular framework that triggers de novo polarisation of the mouse embryo.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1038/s41467-017-00977-8DOIArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5640629/PubMed CentralArticle
ORCID:
AuthorORCID
Zernicka-Goetz, Magdalena0000-0002-7004-2471
Additional Information:© The Author(s) 2017. Open Access - This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. Received: 20 February 2017. Accepted: 9 August 2017. Published 13 October 2017. We thank all colleagues in our lab and D. Glover for the very helpful suggestions on the manuscript. This work was supported by the Wellcome Trust. M.Z.G. is a Wellcome Trust Senior Research Fellow, M.Z. is supported by the Cambridge Trust, and M.N.S. is an EMBO Postdoctoral Fellow. We would like to dedicate this work to M. Johnson, a pioneer of cell polarisation studies. Author Contributions: M.Z. designed and conducted experiments, analysed and interpreted the data with the help of all co-authors. C.Y.L. performed pilot experiments. M.N.S. discussed and helped to interpret the data. M.Z.-G. conceived and supervised the project, and helped to interpret the data. The manuscript was written by M.Z., M.N.S. and M.Z.-G. The authors declare no competing financial interests.
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Funding AgencyGrant Number
Wellcome TrustUNSPECIFIED
Cambridge TrustUNSPECIFIED
European Molecular Biology Organization (EMBO)UNSPECIFIED
PubMed Central ID:PMC5640629
Record Number:CaltechAUTHORS:20190405-170314469
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20190405-170314469
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:94531
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:09 Apr 2019 15:50
Last Modified:03 Oct 2019 21:04

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