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Self-Organizing Properties of Mouse Pluripotent Cells Initiate Morphogenesis upon Implantation

Bedzhov, Ivan and Zernicka-Goetz, Magdalena (2014) Self-Organizing Properties of Mouse Pluripotent Cells Initiate Morphogenesis upon Implantation. Cell, 156 (5). pp. 1032-1044. ISSN 0092-8674. PMCID PMC3991392. doi:10.1016/j.cell.2014.01.023. https://resolver.caltech.edu/CaltechAUTHORS:20190405-170316365

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Abstract

Transformation of pluripotent epiblast cells into a cup-shaped epithelium as the mouse blastocyst implants is a poorly understood and yet key developmental step. Studies of morphogenesis in embryoid bodies led to the current belief that it is programmed cell death that shapes the epiblast. However, by following embryos developing in vivo and in vitro, we demonstrate that not cell death but a previously unknown morphogenetic event transforms the amorphous epiblast into a rosette of polarized cells. This transformation requires basal membrane-stimulated integrin signaling that coordinates polarization of epiblast cells and their apical constriction, a prerequisite for lumenogenesis. We show that basal membrane function can be substituted in vitro by extracellular matrix (ECM) proteins and that ES cells can be induced to form similar polarized rosettes that initiate lumenogenesis. Together, these findings lead to a completely revised model for peri-implantation morphogenesis in which ECM triggers the self-organization of the embryo’s stem cells.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1016/j.cell.2014.01.023DOIArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3991392/PubMed CentralArticle
ORCID:
AuthorORCID
Zernicka-Goetz, Magdalena0000-0002-7004-2471
Additional Information:© 2014 Elsevier Under a Creative Commons license (Attribution 3.0 Unported (CC BY 3.0)) Received 17 October 2013, Revised 24 December 2013, Accepted 9 January 2014, Available online 13 February 2014. We are grateful to David Glover for valuable feedback on the manuscript, Anoeska Van de Moosdijk for the contribution to the ES cell work, and to the Wellcome Trust for providing funding. β1-integrin null and control wild-type ES cells are a kind gift from Reinhard Faessler, Max Planck Institute of Biochemistry.
Funders:
Funding AgencyGrant Number
Wellcome TrustUNSPECIFIED
Issue or Number:5
PubMed Central ID:PMC3991392
DOI:10.1016/j.cell.2014.01.023
Record Number:CaltechAUTHORS:20190405-170316365
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20190405-170316365
Official Citation:Ivan Bedzhov, Magdalena Zernicka-Goetz, Self-Organizing Properties of Mouse Pluripotent Cells Initiate Morphogenesis upon Implantation, Cell, Volume 156, Issue 5, 2014, Pages 1032-1044, ISSN 0092-8674, https://doi.org/10.1016/j.cell.2014.01.023. (http://www.sciencedirect.com/science/article/pii/S0092867414000750)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:94550
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:11 Apr 2019 19:46
Last Modified:16 Nov 2021 17:05

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