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Asymmetric Localization of Cdx2 mRNA during the First Cell-Fate Decision in Early Mouse Development

Skamagki, Maria and Wicher, Krzysztof B. and Jedrusik, Agnieszka and Ganguly, Sujoy and Zernicka-Goetz, Magdalena (2013) Asymmetric Localization of Cdx2 mRNA during the First Cell-Fate Decision in Early Mouse Development. Cell Reports, 3 (2). pp. 442-457. ISSN 2211-1247. PMCID PMC3607255. doi:10.1016/j.celrep.2013.01.006. https://resolver.caltech.edu/CaltechAUTHORS:20190405-170316855

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[img] Video (QuickTime) (Movie S1. Localization of Exogenous Cdx2 mRNA in a Compacted Eight-Cell Embryo, Related to Figure 1) - Supplemental Material
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[img] Video (QuickTime) (Movie S2. Localization of Exogenous Cdx2 mRNA in a 16-Cell-Stage Embryo, Related to Figure 1) - Supplemental Material
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[img] Video (QuickTime) (Movie S3. Cdx2 mRNA Localization during Asymmetric Division of an Eight-Cell Blastomere, Related to Figure 2) - Supplemental Material
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[img] Video (QuickTime) (Movie S4. Cdx2 mRNA Localization during a Symmetric Division of an Eight-Cell Blastomere, Related to Figure 2) - Supplemental Material
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[img] Video (QuickTime) (Movie S5. Persistence of Exogenous WT Cdx2 mRNA at the Cortex, Related to Figure 3) - Supplemental Material
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[img] Video (QuickTime) (Movie S6. Persistence of Exogenous Δ97bp Cdx2 mRNA at the Cortex, Related to Figure 3) - Supplemental Material
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Abstract

A longstanding question in mammalian development is whether the divisions that segregate pluripotent progenitor cells for the future embryo from cells that differentiate into extraembryonic structures are asymmetric in cell-fate instructions. The transcription factor Cdx2 plays a key role in the first cell-fate decision. Here, using live-embryo imaging, we show that localization of Cdx2 transcripts becomes asymmetric during development, preceding cell lineage segregation. Cdx2 transcripts preferentially localize apically at the late eight-cell stage and become inherited asymmetrically during divisions that set apart pluripotent and differentiating cells. Asymmetric localization depends on a cis element within the coding region of Cdx2 and requires cell polarization as well as intact microtubule and actin cytoskeletons. Failure to enrich Cdx2 transcripts apically results in a significant decrease in the number of pluripotent cells. We discuss how the asymmetric localization and segregation of Cdx2 transcripts could contribute to multiple mechanisms that establish different cell fates in the mouse embryo.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1016/j.celrep.2013.01.006DOIArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3607255/PubMed CentralArticle
ORCID:
AuthorORCID
Zernicka-Goetz, Magdalena0000-0002-7004-2471
Additional Information:© 2013 The Authors. Published by Elsevier Under a Creative Commons license (Attribution-NonCommercial-NoDerivs 3.0 Unported (CC BY-NC-ND 3.0)) Received 4 February 2012, Revised 1 June 2012, Accepted 7 January 2013, Available online 31 January 2013. We are grateful to Simon Bullock, David Glover, Claire Chazaud, Rui Pires Martins, Alexander Bruce, Kitai Kim, Isabel Palacios, and Alejandra Gardiol for help and advice. This work was supported by a Wellcome Trust Senior Research Fellowship to M.Z.G. M.S. was funded by the Greek State Scholarships Foundation.
Funders:
Funding AgencyGrant Number
Wellcome TrustUNSPECIFIED
Greek State Scholarships FoundationUNSPECIFIED
Issue or Number:2
PubMed Central ID:PMC3607255
DOI:10.1016/j.celrep.2013.01.006
Record Number:CaltechAUTHORS:20190405-170316855
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20190405-170316855
Official Citation:Maria Skamagki, Krzysztof B. Wicher, Agnieszka Jedrusik, Sujoy Ganguly, Magdalena Zernicka-Goetz, Asymmetric Localization of Cdx2 mRNA during the First Cell-Fate Decision in Early Mouse Development, Cell Reports, Volume 3, Issue 2, 2013, Pages 442-457, ISSN 2211-1247, https://doi.org/10.1016/j.celrep.2013.01.006. (http://www.sciencedirect.com/science/article/pii/S2211124713000132)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:94557
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:23 Apr 2019 17:29
Last Modified:16 Nov 2021 17:05

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