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Concerted cell divisions in embryonic visceral endoderm guide anterior visceral endoderm migration

Antonica, Francesco and Orietti, Lorenzo Carlo and Mort, Richard Lester and Zernicka-Goetz, Magdalena (2019) Concerted cell divisions in embryonic visceral endoderm guide anterior visceral endoderm migration. Developmental Biology, 450 (2). pp. 132-140. ISSN 0012-1606. PMCID PMC6553843. https://resolver.caltech.edu/CaltechAUTHORS:20190408-111045626

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Abstract

Migration of Anterior Visceral Endoderm (AVE) is a critical symmetry breaking event in the early post-implantation embryo development and is essential for establishing the correct body plan. Despite much effort, cellular and molecular events influencing AVE migration are only partially understood. Here, using time-lapse live imaging of mouse embryos, we demonstrate that cell division in the embryonic visceral endoderm is coordinated with AVE migration. Moreover, we demonstrate that temporal inhibition of FGF signalling during the pre-implantation specification of embryonic visceral endoderm perturbs cell cycle progression, thus affecting AVE migration. These findings demonstrate that coordinated cell cycle progression during the implantation stages of development is important for post-implantation morphogenesis in the mouse embryo.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1016/j.ydbio.2019.03.016DOIArticle
http://www.ncbi.nlm.nih.gov/pmc/articles/pmc6553843/PubMed CentralArticle
ORCID:
AuthorORCID
Antonica, Francesco0000-0001-9359-9689
Mort, Richard Lester0000-0002-3566-8217
Zernicka-Goetz, Magdalena0000-0002-7004-2471
Additional Information:© 2019 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). Received 3 December 2018, Revised 26 March 2019, Accepted 26 March 2019, Available online 30 March 2019. The authors declare no competing or financial interests. Author contribution: F.A. designed, performed and analysed all the experiments. L.C.O. helped with some experiments and analysis of data. R.L.M. provided the R26Fucci2a mouse line. F.A. and M.Z.G. conceived the project and wrote the manuscript. This work was supported by Wellcome Trust (098287/Z/12/Z) and European Research Council (669198) grants to M.Z.G., F.A. was supported by an EMBO postdoctoral fellowship and L.C.O. was supported by a Centre for Trophoblast Research PhD studentship. We thank D. Glover, M. N. Shahbazi, N. Christodoulou, C. Kyprianou and A. Cox for discussion and feedback and W. Mansfield for help with embryo transfer.
Funders:
Funding AgencyGrant Number
Wellcome Trust098287/Z/12/Z
European Research Council (ERC)669198
European Molecular Biology Organization (EMBO)UNSPECIFIED
Centre for Trophoblast ResearchUNSPECIFIED
Subject Keywords:4D live imaging; Mouse embryo; Post-implantation morphogenesis; Visceral endoderm
Issue or Number:2
PubMed Central ID:PMC6553843
Record Number:CaltechAUTHORS:20190408-111045626
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20190408-111045626
Official Citation:Francesco Antonica, Lorenzo Carlo Orietti, Richard Lester Mort, Magdalena Zernicka-Goetz, Concerted cell divisions in embryonic visceral endoderm guide anterior visceral endoderm migration, Developmental Biology, Volume 450, Issue 2, 2019, Pages 132-140, ISSN 0012-1606, https://doi.org/10.1016/j.ydbio.2019.03.016. (http://www.sciencedirect.com/science/article/pii/S0012160618307954)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:94562
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:09 Apr 2019 14:43
Last Modified:08 Apr 2020 17:44

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