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Polycystic Ovary Syndrome and Insulin Physiology: An Observational Quantitative Serum Proteomics Study in adolescent, Normal-Weight Females

Manousopoulou, Antigoni and Al-Daghri, Nasser M. and Sabico, Shaun and Garay-Baquero, Diana J. and Teng, Jie and Alenad, Amal and Alokail, Majed S. and Athanasopoulos, Nikos and Deligeoroglou, Efthymios and Chrousos, George P. and Bacopoulou, Flora and Garbis, Spiros D. (2019) Polycystic Ovary Syndrome and Insulin Physiology: An Observational Quantitative Serum Proteomics Study in adolescent, Normal-Weight Females. Proteomics - Clinical Applications, 13 (5). Art. No. 1800184. ISSN 1862-8346. https://resolver.caltech.edu/CaltechAUTHORS:20190415-083927122

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Abstract

Background: Polycystic ovary syndrome (PCOS) is a common endocrine disorder associated with insulin resistance, even in the absence of overweight/obesity. The aim of the present study is to examine the global serum proteomic profile of adolescent, normal‐weight females with PCOS in order to gain novel insight in the association of this endocrine disorder with insulin physiology and to identify novel circulating markers that can guide intervention protocols. Methods: Non‐depleted serum from normal‐weight (BMI: 18–23 kg m^(−2)), adolescent females (13–21 years old) with PCOS (n = 20) is compared to BMI‐ and age‐matched healthy controls (n = 20) using our 3D quantitative proteomics methodology. Serum samples from study participants are randomly pooled to form four biological replicates of females with PCOS and four of healthy controls (n = 5 per sample pool). Results: One‐hundred and twenty‐six proteins are differentially expressed in females with PCOS compared to controls. Gene ontology analysis shows significant enrichment for terms related to inflammatory immune response, metabolism and insulin‐like growth factor receptor signaling pathway. Circulating levels of IGF‐1 and ‐2 and IGFBP‐2, ‐3, and ‐4 are found to be lower in females with PCOS compared to healthy controls. Conclusions: The present serum proteomics study provides insight into the pro‐inflammatory status and insulin dysregulation in young females with PCOS and identifies potential serological markers that can guide early intervention protocols.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1002/prca.201800184DOIArticle
ORCID:
AuthorORCID
Manousopoulou, Antigoni0000-0001-5028-1865
Garbis, Spiros D.0000-0002-1050-0805
Additional Information:© 2019 The Authors. Proteomics – Clinical Application published by WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. Issue Online: 18 September 2019; Version of Record online: 23 April 2019; Accepted manuscript online: 09 April 2019; Manuscript revised: 12 February 2019; Manuscript received: 15 November 2018. A.M. and N.M.A. have contributed equally to this work. Concept and clinical design: A.M., N.M.A., G.P.C., F.B., and S.D.G.; Proteomics analysis: A.M., D.J.G., J.T., A.A., and S.D.G.; Sample procurement and initial evaluation: G.P.C. and F.B.; Data interpretation: A.M., G.P.C., F.B., and S.D.G.; Guided the design and performance of specific aspects of the experiments: M.S.A., N.A., E.D., G.P.C., and F.B.; Wrote the manuscript: A.M., F.B., and S.D.G.; all authors contributed to its revision and final form. This study was financially supported by the Deanship of Scientific Research, Chair for Biomarkers of Chronic Diseases, in King Saud University, Riyadh, Saudi Arabia; the Wessex Cancer Trust, Wessex Medical Research; EU‐FP7/HELLENIC NSRF (BIOEXPLORE); Annual Adventures in Research—University of Southampton; and the EU‐Excellence II—Systems Biology Framework FRA‐SYS (Grant 4072). The authors declare no conflict of interest.
Funders:
Funding AgencyGrant Number
King Saud UniversityUNSPECIFIED
Wessex Cancer TrustUNSPECIFIED
Wessex Medical ResearchUNSPECIFIED
European Research Council (ERC)BIOEXPLORE
University of SouthamptonUNSPECIFIED
European Union4072
Subject Keywords:inflammation; insulin resistance; iTRAQ; non‐depleted serum; PCOS; proteomics
Issue or Number:5
Record Number:CaltechAUTHORS:20190415-083927122
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20190415-083927122
Official Citation:Manousopoulou, A., Al‐Daghri, N. M., Sabico, S., Garay‐Baquero, D. J., Teng, J., Alenad, A., Alokail, M. S., Athanasopoulos, N., Deligeoroglou, E., Chrousos, G. P., Bacopoulou, F., Garbis, S. D., Polycystic Ovary Syndrome and Insulin Physiology: An Observational Quantitative Serum Proteomics Study in Adolescent, Normal‐Weight Females. Prot. Clin. Appl. 2019, 13, 1800184. https://doi.org/10.1002/prca.201800184
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:94698
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:16 Apr 2019 21:52
Last Modified:03 Oct 2019 21:06

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