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Electron Cryotomography of Bacterial Secretion Systems

Oikonomou, Catherine M. and Jensen, Grant J. (2019) Electron Cryotomography of Bacterial Secretion Systems. Microbiology Spectrum, 7 (2). Art. No. PSIB-0019. ISSN 2165-0497. PMCID PMC6452891. https://resolver.caltech.edu/CaltechAUTHORS:20190415-141326985

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Abstract

In biology, function arises from form. For bacterial secretion systems, which often span two membranes, avidly bind to the cell wall, and contain hundreds of individual proteins, studying form is a daunting task, made possible by electron cryotomography (ECT). ECT is the highest-resolution imaging technique currently available to visualize unique objects inside cells, providing a three-dimensional view of the shapes and locations of large macromolecular complexes in their native environment. Over the past 15 years, ECT has contributed to the study of bacterial secretion systems in two main ways: by revealing intact forms for the first time and by mapping components into these forms. Here we highlight some of these contributions, revealing structural convergence in type II secretion systems, structural divergence in type III secretion systems, unexpected structures in type IV secretion systems, and unexpected mechanisms in types V and VI secretion systems. Together, they offer a glimpse into a world of fantastic forms—nanoscale rotors, needles, pumps, and dart guns—much of which remains to be explored.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1128/microbiolspec.PSIB-0019-2018DOIArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6452891/PubMed CentralArticle
ORCID:
AuthorORCID
Oikonomou, Catherine M.0000-0003-2312-4746
Jensen, Grant J.0000-0003-1556-4864
Additional Information:© 2019 American Society for Microbiology. Received: 19 October 2018, Accepted: 11 February 2019, Published: 5 April 2019. We apologize to our colleagues whose work we could not discuss due to limited space, and we thank members of the Jensen lab for helpful discussions. Work on bacterial secretion systems in the lab is supported by the NIH (grant R01 AI127401 to G.J.J.).
Funders:
Funding AgencyGrant Number
NIHR01 AI127401
Issue or Number:2
PubMed Central ID:PMC6452891
Record Number:CaltechAUTHORS:20190415-141326985
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20190415-141326985
Official Citation:Oikonomou CM, Jensen GJ. 2019. Electron cryotomography of bacterial secretion systems. Microbiol Spectrum 7(2):PSIB-0019-2018. doi:10.1128/microbiolspec.PSIB-0019-2018
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:94711
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:16 Apr 2019 21:35
Last Modified:03 Oct 2019 21:06

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