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Developmental Plasticity Is Bound by Pluripotency and the Fgf and Wnt Signaling Pathways

Morris, Samantha A. and Guo, Yu and Zernicka-Goetz, Magdalena (2012) Developmental Plasticity Is Bound by Pluripotency and the Fgf and Wnt Signaling Pathways. Cell Reports, 2 (4). pp. 756-765. ISSN 2211-1247. PMCID PMC3607220. doi:10.1016/j.celrep.2012.08.029. https://resolver.caltech.edu/CaltechAUTHORS:20190417-152709752

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[img] Video (QuickTime) (Movie S1. Time-Lapse Imaging of Half and Whole Embryos, Related to Figure 1) - Supplemental Material
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[img] Video (QuickTime) (Movie S2. Developmental Schedule of Half and Whole Embryos, Related to Figure 1.) - Supplemental Material
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[img] Video (QuickTime) (Movie S3. High-resolution time-lapse confocal imaging of a half embryo injected with GAP43-RFP to visualize cell membranes) - Supplemental Material
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[img] Video (QuickTime) (Movie S4. Cell Internalization by Asymmetric Division in Whole Embryos, Related to Figure 1) - Supplemental Material
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Abstract

Plasticity is a well-known feature of mammalian development, and yet very little is known about its underlying mechanism. Here, we establish a model system to examine the extent and limitations of developmental plasticity in living mouse embryos. We show that halved embryos follow the same strict clock of developmental transitions as intact embryos, but their potential is not equal. We have determined that unless a minimum of four pluripotent cells is established before implantation, development will arrest. This failure can be rescued by modulating Fgf and Wnt signaling to enhance pluripotent cell number, allowing the generation of monozygotic twins, which is an otherwise rare phenomenon. Knowledge of the minimum pluripotent-cell number required for development to birth, as well as the different potentials of blastomeres, allowed us to establish a protocol for splitting an embryo into one part that develops to adulthood and another that provides embryonic stem cells for that individual.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1016/j.celrep.2012.08.029DOIArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3607220/PubMed CentralArticle
ORCID:
AuthorORCID
Zernicka-Goetz, Magdalena0000-0002-7004-2471
Additional Information:© 2012 The Authors. Published by Elsevier Under a Creative Commons license (Attribution 3.0 Unported (CC BY 3.0)) Received 9 July 2012, Revised 15 August 2012, Accepted 27 August 2012, Available online 4 October 2012. We thank Lewis Wolpert for the inspiration for this study; Sean Jeffries, Harry Leitch, and David Glover for discussions; Emma Rawlins for help with imaging the E11.5 embryos; and the M.Z.G. group for support. This work was supported by a Wellcome Trust grant to M.Z.G.
Funders:
Funding AgencyGrant Number
Wellcome TrustUNSPECIFIED
Issue or Number:4
PubMed Central ID:PMC3607220
DOI:10.1016/j.celrep.2012.08.029
Record Number:CaltechAUTHORS:20190417-152709752
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20190417-152709752
Official Citation:Samantha A. Morris, Yu Guo, Magdalena Zernicka-Goetz, Developmental Plasticity Is Bound by Pluripotency and the Fgf and Wnt Signaling Pathways, Cell Reports, Volume 2, Issue 4, 2012, Pages 756-765, ISSN 2211-1247, https://doi.org/10.1016/j.celrep.2012.08.029. (http://www.sciencedirect.com/science/article/pii/S2211124712002690)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:94758
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:18 Apr 2019 14:28
Last Modified:16 Nov 2021 17:07

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