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Phospholipase C-ζ-induced Ca^(2+) oscillations cause coincident cytoplasmic movements in human oocytes that failed to fertilize after intracytoplasmic sperm injection

Swann, Karl and Windsor, Shane and Campbell, Karen and Elgmati, Khalil and Nomikos, Michail and Zernicka-Goetz, Magdalena and Amso, Nazar and Lai, F. Anthony and Thomas, Adrian and Graham, Christopher (2012) Phospholipase C-ζ-induced Ca^(2+) oscillations cause coincident cytoplasmic movements in human oocytes that failed to fertilize after intracytoplasmic sperm injection. Fertility and Sterility, 97 (3). pp. 742-747. ISSN 0015-0282. PMCID PMC3334266. doi:10.1016/j.fertnstert.2011.12.013. https://resolver.caltech.edu/CaltechAUTHORS:20190417-152709920

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Abstract

OBJECTIVE: To evaluate the imaging of cytoplasmic movements in human oocytes as a potential method to monitor the pattern of Ca^(2+) oscillations during activation. DESIGN: Test of a laboratory technique. SETTING: University medical school research laboratory. PATIENT(S): Donated unfertilized human oocytes from intracytoplasmic sperm injection (ICSI) cycles. INTERVENTION(S): Microinjection of oocytes with phospholipase C (PLC) zeta (ζ) cRNA and a Ca^(2+)-sensitive fluorescent dye. MAIN OUTCOME MEASURE(S): Simultaneous detection of oocyte cytoplasmic movements using particle image velocimetry (PIV) and of Ca^(2+) oscillations using a Ca^(2+)-sensitive fluorescent dye. RESULT(S): Microinjection of PLCζ cRNA into human oocytes that had failed to fertilize after ICSI resulted in the appearance of prolonged Ca^(2+) oscillations. Each transient Ca^(2+) concentration change was accompanied by a small coordinated movement of the cytoplasm that could be detected using PIV analysis. CONCLUSION(S): The occurrence and frequency of cytoplasmic Ca^(2+) oscillations, a critical parameter in activating human zygotes, can be monitored by PIV analysis of cytoplasmic movements. This simple method provides a novel, noninvasive approach to determine in real time the occurrence and frequency of Ca^(2+) oscillations in human zygotes.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1016/j.fertnstert.2011.12.013DOIArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3334266/PubMed CentralArticle
ORCID:
AuthorORCID
Zernicka-Goetz, Magdalena0000-0002-7004-2471
Alternate Title:Phospholipase C-ζ-induced Ca2+ oscillations cause coincident cytoplasmic movements in human oocytes that failed to fertilize after intracytoplasmic sperm injection
Additional Information:© 2012 American Society for Reproductive Medicine. Published by Elsevier Under a Creative Commons license (Attribution 3.0 Unported (CC BY 3.0)) Received 18 October 2011, Revised 6 December 2011, Accepted 9 December 2011, Available online 2 January 2012. K.S. has nothing to disclose. S.W. has nothing to disclose. K.C. has nothing to disclose. K.E. has nothing to disclose. M.N. has nothing to disclose. M.Z.-G. has nothing to disclose. N.A. has nothing to disclose. F.A.L. has nothing to disclose. A.T. has nothing to disclose. C.G. has nothing to disclose. This work was supported by Wellcome Trust grants awarded to A.T., M.Z.-G., and K.S. (085107/Z/08/Z) and to K.S. and F.A.L. (080701/Z/06/Z). K.E. is supported by a Scholarship from the Libyan Government.
Funders:
Funding AgencyGrant Number
Wellcome Trust085107/Z/08/Z
Wellcome Trust080701/Z/06/Z
Libyan GovernmentUNSPECIFIED
Subject Keywords:Oocyte; zygote; calcium; movement; phospholipase C zeta; cross correlation; particle image velocimetry
Issue or Number:3
PubMed Central ID:PMC3334266
DOI:10.1016/j.fertnstert.2011.12.013
Record Number:CaltechAUTHORS:20190417-152709920
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20190417-152709920
Official Citation:Karl Swann, Shane Windsor, Karen Campbell, Khalil Elgmati, Michail Nomikos, Magdalena Zernicka-Goetz, Nazar Amso, F. Anthony Lai, Adrian Thomas, Christopher Graham, Phospholipase C-ζ-induced Ca2+ oscillations cause coincident cytoplasmic movements in human oocytes that failed to fertilize after intracytoplasmic sperm injection, Fertility and Sterility, Volume 97, Issue 3, 2012, Pages 742-747, ISSN 0015-0282, https://doi.org/10.1016/j.fertnstert.2011.12.013. (http://www.sciencedirect.com/science/article/pii/S0015028211028652)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:94760
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:17 Apr 2019 23:01
Last Modified:16 Nov 2021 17:07

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