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Dynamics of anterior–posterior axis formation in the developing mouse embryo

Morris, Samantha A. and Grewal, Seema and Barrios, Florencia and Patankar, Sameer N. and Strauss, Bernhard and Buttery, Lee and Alexander, Morgan and Shakesheff, Kevin M. and Zernicka-Goetz, Magdalena (2012) Dynamics of anterior–posterior axis formation in the developing mouse embryo. Nature Communications, 3 . Art. No. 673. ISSN 2041-1723. PMCID PMC3293425. https://resolver.caltech.edu/CaltechAUTHORS:20190417-152710020

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[img] Video (QuickTime) (Supplementary Movie 3. Live imaging of a representative embryo throughout development in vitro, from the blastocyst to egg cylinder stage) - Supplemental Material
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[img] Video (QuickTime) (Supplementary Movie 4. Time-lapse confocal imaging of development of chimeric embryos, on collagen-coated hydrogels, from the implanting blastocyst stage (late Day 2) to the egg cylinder stage (Day 4)) - Supplemental Material
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[img] Video (QuickTime) (Supplementary Movie 5. Live imaging of a representative Cerl-GFP embryo throughout development in vitro, from the blastocyst to the egg cylinder stage) - Supplemental Material
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[img] Video (QuickTime) (Supplementary Movie 6. Cerl-GFP embryo developing on a collagen-coated polyacrylamide matrix from the blastocyst to the egg cylinder stage) - Supplemental Material
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[img] Video (QuickTime) (Supplementary Movie 7. Live imaging of AVE migration in control embryos expressing Cerl- GFP and recovered from mothers at E5.5) - Supplemental Material
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[img] Video (QuickTime) (Supplementary Movie 8. Live imaging of Cerl-GFP expressing embryos in which the pioneer cell was ablated at E5.5) - Supplemental Material
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Abstract

The development of an anterior–posterior (AP) polarity is a crucial process that in the mouse has been very difficult to analyse, because it takes place as the embryo implants within the mother. To overcome this obstacle, we have established an in-vitro culture system that allows us to follow the step-wise development of anterior visceral endoderm (AVE), critical for establishing AP polarity. Here we use this system to show that the AVE originates in the implanting blastocyst, but that additional cells subsequently acquire AVE characteristics. These 'older' and 'younger' AVE domains coalesce as the egg cylinder emerges from the blastocyst structure. Importantly, we show that AVE migration is led by cells expressing the highest levels of AVE marker, highlighting that asymmetry within the AVE domain dictates the direction of its migration. Ablation of such leading cells prevents AVE migration, suggesting that these cells are important for correct establishment of the AP axis.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1038/ncomms1671DOIArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3293425/PubMed CentralArticle
ORCID:
AuthorORCID
Zernicka-Goetz, Magdalena0000-0002-7004-2471
Additional Information:© 2012 Macmillan Publishers Limited. This work is licensed under a Creative Commons Attribution-NonCommercial-Share Alike 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/ Received 20 October 2011. Accepted 09 January 2012. Published 14 February 2012. We are grateful to Professor David Glover for his comments on the manuscript, Professor Azim Surani and Caroline Lee for kindly providing Oct4ΔPE-GFP mice, and Harry Leitch for providing the dsRed ES cells. F. B. holds a Newton International Fellowship from the Royal Society, which supported this work. This work was supported by a Wellcome Trust Senior Fellowship to M. Z. G. Samantha A. Morris, Seema Grewal & Florencia Barrios: These authors contributed equally to this work. Author Contributions: S. A. M. performed experiments on AVE migration, analysed data and assisted in the interpretation of these results. S. G. developed the embryo culture method, and together with F. B., cultured and experimented on the embryos from pre- to post-implantation stages and assisted in the interpretation of the results. L. B., M. A. and K. M. S. designed substrates for the embryo culture, and S. N. P. built the gels and demonstrated their applicability. K. M. S. assisted in preparing the manuscript. M. Z. G. designed the experiments, interpreted the results and wrote the manuscript. The authors declare no competing financial interests.
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Royal SocietyUNSPECIFIED
Wellcome TrustUNSPECIFIED
PubMed Central ID:PMC3293425
Record Number:CaltechAUTHORS:20190417-152710020
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20190417-152710020
Official Citation:Morris, S. A. et al. Dynamics of anterior – posterior axis formation in the developing mouse embryo. Nat. Commun. 3:673 doi:10.1038 / ncomms1671 (2012).
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:94761
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:17 Apr 2019 22:58
Last Modified:03 Oct 2019 21:06

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