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Rhythmic actomyosin-driven contractions induced by sperm entry predict mammalian embryo viability

Ajduk, Anna and Ilozue, Tagbo and Windsor, Shane and Yu, Yuansong and Seres, K. Bianka and Bomphrey, Richard J. and Tom, Brian D. and Swann, Karl and Thomas, Adrian and Graham, Chris and Zernicka-Goetz, Magdalena (2011) Rhythmic actomyosin-driven contractions induced by sperm entry predict mammalian embryo viability. Nature Communications, 2 . Art. No. 417. ISSN 2041-1723. PMCID PMC3265380. doi:10.1038/ncomms1424. https://resolver.caltech.edu/CaltechAUTHORS:20190417-163114215

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Abstract

Fertilization-induced cytoplasmic flows are a conserved feature of eggs in many species. However, until now the importance of cytoplasmic flows for the development of mammalian embryos has been unknown. Here, by combining a rapid imaging of the freshly fertilized mouse egg with advanced image analysis based on particle image velocimetry, we show that fertilization induces rhythmical cytoplasmic movements that coincide with pulsations of the protrusion forming above the sperm head. We find that these movements are caused by contractions of the actomyosin cytoskeleton triggered by Ca^(2+) oscillations induced by fertilization. Most importantly, the relationship between the movements and the events of egg activation makes it possible to use the movements alone to predict developmental potential of the zygote. In conclusion, this method offers, thus far, the earliest and fastest, non-invasive way to predict the viability of eggs fertilized in vitro and therefore can potentially improve greatly the prospects for IVF treatment.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1038/ncomms1424DOIArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3265380/PubMed CentralArticle
ORCID:
AuthorORCID
Zernicka-Goetz, Magdalena0000-0002-7004-2471
Additional Information:© 2011 Macmillan Publishers Limited. This work is licensed under a Creative Commons Attribution-NonCommercial-Share Alike 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/ Received 18 February 2011. Accepted 07 June 2011. Published 09 August 2011. We thank Drs Melina Schuh for UtrCH–EGFP; Ewa Paluch for MyoRLC–GFP constructs; William Mansfield for his help with embryo transfers; and David Glover, Krzysztof Wicher, Samantha Morris, Kevin Coward and Celine Jones for their advice and support. The work was funded by the Wellcome Trust with grants to M.Z.-G., T.I., K.S. and A.T. A.A. was a recipient of the FEBS Short-Term Fellowship and of 'START' and 'KOLUMB' fellowships from the Foundation for Polish Science. T.I. was supported by the AstraZeneca funding for MB/PhD students. R.J.B. holds an EPSRC Career Acceleration Fellowship and was supported during this work by the BBSRC. K.B.S. was supported by the Oxford MSc Programme in Clinical Embryology. B.D.T. was funded by the UK Medical Research Council under the Programme Grant MC_US_A030_0022. Author Contributions: All the data was collected in M.Z.-G's laboratory and most data in this paper is the result of experiments conducted by A.A., T.I., Y.Y. and K.B.S. with image processing carried out by S.W., R.J.B. and A.A,. and statistical analysis by B.D.T. The other authors provided extensive advice over a long period of collaboration. The paper was written by A.A. and M.Z.-G, with some methodological sections revised by S.W. and B.D.T. Competing interests: A U.S. patent has been filed on this work. 'Methods for predicting mammalian embryo viability' (US application no. 61/503827).
Funders:
Funding AgencyGrant Number
Wellcome TrustUNSPECIFIED
Federation of the European Biochemical Societies (FEBS)UNSPECIFIED
Foundation for Polish ScienceUNSPECIFIED
AstraZenecaUNSPECIFIED
Engineering and Physical Sciences Research Council (EPSRC)UNSPECIFIED
Biotechnology and Biological Sciences Research Council (BBSRC)UNSPECIFIED
University of OxfordUNSPECIFIED
Medical Research Council (UK)MC_US_A030_0022
PubMed Central ID:PMC3265380
DOI:10.1038/ncomms1424
Record Number:CaltechAUTHORS:20190417-163114215
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20190417-163114215
Official Citation:Ajduk, A. et al. Rhythmic actomyosin-driven contractions induced by sperm entry predict mammalian embryo viability. Nat. Commun. 2:417 doi: 10.1038/ncomms1424 (2011).
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:94766
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:18 Apr 2019 22:54
Last Modified:16 Nov 2021 17:07

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