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Novel gene expression patterns along the proximo-distal axis of the mouse embryo before gastrulation

Frankenberg, Stephen and Smith, Lee and Greenfield, Andy and Zernicka-Goetz, Magdalena (2007) Novel gene expression patterns along the proximo-distal axis of the mouse embryo before gastrulation. BMC Developmental Biology, 7 . Art. No. 8. ISSN 1471-213X. PMCID PMC1821012. https://resolver.caltech.edu/CaltechAUTHORS:20190418-150056179

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Abstract

Background: To date, the earliest stage at which the orientation of the anterior-posterior axis in the mouse embryo is distinguishable by asymmetric gene expression is shortly after E5.5. At E5.5, prospective anterior markers are expressed at the distal tip of the embryo, whereas prospective posterior markers are expressed more proximally, close to the boundary with the extraembryonic region. Results: To contribute to elucidating the mechanisms underlying the events involved in early patterning of the mouse embryo, we have carried out a microarray screen to identify novel genes that are differentially expressed between the distal and proximal parts of the E5.5 embryo. Secondary screening of resulting candidates by in situ hybridisation at E5.5 and E6.5 revealed novel expression patterns for known and previously uncharacterised genes, including Peg10, Ctsz1, Cubilin, Jarid1b, Ndrg1, Sfmbt2, Gjb5, Talia and Plet1. The previously undescribed gene Talia and recently identified Plet1 are expressed specifically in the distal-most part of the extraembryonic ectoderm, adjacent to the epiblast, and are therefore potential candidates for regulating early patterning events. Talia and the previously described gene XE7 define a gene family highly conserved among metazoans and with a predicted protein structure suggestive of a post-transcriptional regulative function, whilst Plet1 appears to be mammal-specific and of unknown function. Conclusion: Our approach has allowed us to compare expression between dissected parts of the egg cylinder and has identified multiple genes with novel expression patterns at this developmental stage. These genes are potential candidates for regulating tissue interactions following implantation.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1186/1471-213x-7-8DOIArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1821012/PubMed CentralArticle
ORCID:
AuthorORCID
Zernicka-Goetz, Magdalena0000-0002-7004-2471
Additional Information:© Frankenberg et al; licensee BioMed Central Ltd. 2007. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Received: 02 November 2006. Accepted: 15 February 2007. Published: 15 February 2007. This work was funded by Human Frontiers Scientific Programme and Wellcome Trust grants to MZG. MZG also thanks the Wellcome Trust for her Senior Research Fellowship. The microarrays used in this study were made available free of charge by the HGMP Resource Centre, Hinxton, UK. We thank Dr Mark DePristo for assistance in protein structure analysis and Dr Claire Chazaud for support during the latter part of the study. Authors' contributions: SF performed all collection and experimental work on mouse embryos and writing of the manuscript. SF, with the crucial assistance of LS, performed the microarray screen and data analysis. AG was involved in facilitating and coordinating the microarray experiments. MZG was involved in the conception and coordination of this project. All authors approved the final manuscript.
Funders:
Funding AgencyGrant Number
Human Frontier Science ProgramUNSPECIFIED
Wellcome TrustUNSPECIFIED
Subject Keywords:Imprint Gene; Primitive Streak; Visceral Endoderm; Ectoplacental Cone; Extraembryonic Ectoderm
PubMed Central ID:PMC1821012
Record Number:CaltechAUTHORS:20190418-150056179
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20190418-150056179
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:94802
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:18 Apr 2019 23:09
Last Modified:03 Oct 2019 21:07

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