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Dishevelled proteins regulate cell adhesion in mouse blastocyst and serve to monitor changes in Wnt signaling

Na, Jie and Lykke-Andersen, Karin and Torres Padilla, Maria Elena and Zernicka-Goetz, Magdalena (2007) Dishevelled proteins regulate cell adhesion in mouse blastocyst and serve to monitor changes in Wnt signaling. Developmental Biology, 302 (1). pp. 40-49. ISSN 0012-1606. PMCID PMC3353122. doi:10.1016/j.ydbio.2006.08.036. https://resolver.caltech.edu/CaltechAUTHORS:20190418-150056272

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Abstract

Wnt signaling is essential for the regulation of cell polarity and cell fate in the early embryogenesis of many animal species. Multiple Wnt genes and its pathway members are expressed in the mouse early embryo, raising the question whether they play any roles in preimplantation development. Dishevelled is an important transducer of divergent Wnt pathways. Here we show that three of the mouse Dishevelled proteins are not only expressed in oocytes and during preimplantation development, but also display distinct spatio-temporal localization. Interestingly, as embryos reach blastocyst stage, Dishevelled 2 becomes increasingly associated with cell membrane in trophectoderm cells, while at E4.5, Dishevelled 3 is highly enriched in the cytoplasm of ICM cells. These changes are coincident with an increase in the active form of β-catenin, p120catenin transcription and decrease of Kaiso expression, indicating an upregulation of Wnt signaling activity before implantation. When Dishevelled-GFP fusion proteins are overexpressed in single blastomeres of the 4-cell stage embryo, the progeny of this cell show reduction in cell adhesiveness and a rounded shape at the blastocyst stage. This suggests that perturbing Dvl function interferes with cell–cell adhesion through the non-canonical Wnt pathway in blastocysts.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1016/j.ydbio.2006.08.036DOIArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3353122/PubMed CentralArticle
ORCID:
AuthorORCID
Zernicka-Goetz, Magdalena0000-0002-7004-2471
Additional Information:© 2006 Elsevier. Under an Elsevier user license. Received 26 July 2006, Accepted 15 August 2006, Available online 22 August 2006. We are grateful to the Wellcome Trust for a Senior Research Fellowship and BBSRC grant to MZG which both supported this work. Jie Na is currently supported by a Royal Society Relocation Fellowship. We thank Daniel Sussman for mouse Dishevelled 1, 2 and 3 cDNA, Lin Mei for Dvl1 antibody, Randall Moon for initial discussion of this project, Jerome Jullien and Ilenia Simeoni in John Gurdon's laboratory for assistance in injection of Xenopus embryos, and Sigolène M. Meilhac for assistance in BAT-gal embryo staining.
Funders:
Funding AgencyGrant Number
Wellcome TrustUNSPECIFIED
Biotechnology and Biological Sciences Research Council (BBSRC)UNSPECIFIED
Royal SocietyUNSPECIFIED
Subject Keywords:Dishevelled; Mouse; Blastocyst; Preimplantation; Development
Issue or Number:1
PubMed Central ID:PMC3353122
DOI:10.1016/j.ydbio.2006.08.036
Record Number:CaltechAUTHORS:20190418-150056272
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20190418-150056272
Official Citation:Jie Na, Karin Lykke-Andersen, Maria Elena Torres Padilla, Magdalena Zernicka-Goetz, Dishevelled proteins regulate cell adhesion in mouse blastocyst and serve to monitor changes in Wnt signaling, Developmental Biology, Volume 302, Issue 1, 2007, Pages 40-49, ISSN 0012-1606, https://doi.org/10.1016/j.ydbio.2006.08.036. (http://www.sciencedirect.com/science/article/pii/S0012160606011067)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:94803
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:18 Apr 2019 23:00
Last Modified:16 Nov 2021 17:07

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