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Dynamic distribution of the replacement histone variant H3.3 in the mouse oocyte and preimplantation embryos

Torres-Padilla, Maria-Elena and Bannister, Andrew J. and Hurd, Paul J. and Kouzarides, Tony and Zernicka-Goetz, Magdalena (2006) Dynamic distribution of the replacement histone variant H3.3 in the mouse oocyte and preimplantation embryos. International Journal of Developmental Biology, 50 (5). pp. 455-461. ISSN 0214-6282. doi:10.1387/ijdb.052073mt. https://resolver.caltech.edu/CaltechAUTHORS:20190419-105737033

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Abstract

Upon fertilization, the gametes undergo a drastic reprogramming that includes changes in DNA methylation and histone modifications. Currently, it is not known whether replacement of the major histones by histone variants is also involved in these processes. Here we have examined the expression and localization of the histone variant H3.3 in early mouse embryogenesis. We show that H3.3 is present in the oocyte as a maternal factor. It is then incorporated preferentially into the male pronucleus before genome activation, pointing towards an asymmetry in histone composition between the two pronuclei. This is in line with the male pronucleus bearing transcriptional activation first. The same distribution was observed when we followed the localisation of a tagged version of H3.3. We detected H3.3 in the nuclei of mouse embryos in all of the stages analysed, from the zygote to the blastocyst stage, suggesting that the epigenetic mechanisms in the early embryo not only involve changes in histone modifications but may also include histone replacement.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1387/ijdb.052073mtDOIArticle
ORCID:
AuthorORCID
Zernicka-Goetz, Magdalena0000-0002-7004-2471
Additional Information:© 2006 UBC Press. Received: July 2005. Reviewed by Referees: September 2005. Modified by Authors and Accepted for Publication: January 2006. This work was supported by the Wellcome Trust. M.Z-G. is a Wellcome Senior Research Fellow. M-E.-T-P. is a Long Term EMBO fellow.
Funders:
Funding AgencyGrant Number
Wellcome TrustUNSPECIFIED
European Molecular Biology Organization (EMBO)UNSPECIFIED
Subject Keywords:histone variant, early mouse embryo, reprogramming, replacement histone
Issue or Number:5
DOI:10.1387/ijdb.052073mt
Record Number:CaltechAUTHORS:20190419-105737033
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20190419-105737033
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:94813
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:23 Apr 2019 17:06
Last Modified:16 Nov 2021 17:08

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