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Spatial arrangement of individual 4-cell stage blastomeres and the order in which they are generated correlate with blastocyst pattern in the mouse embryo

Piotrowska-Nitsche, Karolina and Zernicka-Goetz, Magdalena (2005) Spatial arrangement of individual 4-cell stage blastomeres and the order in which they are generated correlate with blastocyst pattern in the mouse embryo. Mechanisms of Development, 122 (4). pp. 487-500. ISSN 0925-4773. doi:10.1016/j.mod.2004.11.014. https://resolver.caltech.edu/CaltechAUTHORS:20190419-105737708

[img] Image (TIFF) (Fig. S1. Exceptional embryos with most of their embryonic or abembryonic parts derived from one or other of the 2-cell stage blastomeres in which the tilt between the clonal border (as we are able to define it) and the boundary zone is higher than in the ) - Supplemental Material
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[img] PDF (Table S1) - Supplemental Material
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[img] Video (QuickTime) (Movie 1. The first cleavage in a series of 10 zygotes in which the polar body (PB) lies within 30° of the cleavage plane in seven cases) - Supplemental Material
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[img] Video (QuickTime) (Movie 2. Time-lapse movie of a series of 2-cell stage embryos in which the cell not attached to the PB has been destroyed) - Supplemental Material
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[img] Video (QuickTime) (Movie 3 (3 optical planes)) - Supplemental Material
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[img] Video (QuickTime) (Movie 3 (3 optical planes)) - Supplemental Material
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[img] Video (QuickTime) (Movie 3 (3 optical planes)) - Supplemental Material
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Abstract

In the unperturbed development of the mouse embryo one of the 2-cell blastomeres tends to contribute its progeny predominantly to the embryonic and the other to the abembryonic part of the blastocyst. However, a significant minority of embryos (20–30%) do not show this correlation. In this study, we have used non-invasive lineage tracing to determine whether development of blastocyst pattern shows any correlation with the orientation and order of the second cleavage divisions that result in specific positioning of blastomeres at the 4-cell stage. Although the orientation and order of the second cleavages are not predetermined, in the great majority (80%) of embryos the spatial arrangement of 4-cell blastomeres is consistent with one of the second cleavages occurring meridionally and the other equatorially or obliquely with respect to the polar body. In such cleaving embryos, one of the 2-cell stage blastomeres tends to contribute to embryonic while the other contributes predominantly to abembryonic part of the blastocyst. Thus, in these embryos the outcome of the first cleavage tends to correlate with the orientation of the blastocyst embryonic–abembryonic axis. However, the order of blastomere divisions predicts a specific polarity for this axis only when the earlier 2-cell blastomere to divide does so meridionally. In contrast to the above two groups, in those embryos in which both second cleavage divisions occur in a similar orientation, either meridionally or equatorially, we do not observe any tendency for the 2-cell blastomeres to contribute to specific blastocyst parts. We find that all these groups of embryos develop to term with similar success, with the exception of those in which both second cleavage divisions occur equatorially whose development can be compromised. We conclude that the orientations and order of the second cleavages are not predetermined; they correlate with the development of blastocyst patterning; and that the majority, but not all, of these cleavage patterns allow equally successful development.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1016/j.mod.2004.11.014DOIArticle
ORCID:
AuthorORCID
Zernicka-Goetz, Magdalena0000-0002-7004-2471
Additional Information:© 2004 Elsevier Under an Elsevier user license. Received 25 October 2004, Revised 24 November 2004, Accepted 24 November 2004, Available online 18 December 2004. The work was supported by the Wellcome Trust Senior Fellowship to MZG. KPN is a Marie Curie Fellow. We thank David Glover, Jonathan Pines, John Gurdon, Anne McLaren, Aitana Perea-Gomez and Stephen Frankenberg for helpful discussions, their support and comments on the manuscript. We also thank Dionne Gray for time-lapse movies of the first cleavage division.
Funders:
Funding AgencyGrant Number
Wellcome TrustUNSPECIFIED
Marie Curie FellowshipUNSPECIFIED
Subject Keywords:Blastocyst formation; Cleavage pattern; Cell fate; Mouse embryo
Issue or Number:4
DOI:10.1016/j.mod.2004.11.014
Record Number:CaltechAUTHORS:20190419-105737708
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20190419-105737708
Official Citation:Karolina Piotrowska-Nitsche, Magdalena Zernicka-Goetz, Spatial arrangement of individual 4-cell stage blastomeres and the order in which they are generated correlate with blastocyst pattern in the mouse embryo, Mechanisms of Development, Volume 122, Issue 4, 2005, Pages 487-500, ISSN 0925-4773, https://doi.org/10.1016/j.mod.2004.11.014. (http://www.sciencedirect.com/science/article/pii/S0925477304002795)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:94817
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:23 Apr 2019 16:48
Last Modified:16 Nov 2021 17:08

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