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The first cleavage of the mouse zygote predicts the blastocyst axis

Plusa, Berenika and Hadjantonakis, Anna-Katerina and Gray, Dionne and Piotrowska-Nitsche, Karolina and Jedrusik, Agnieszka and Papaioannou, Virginia E. and Glover, David M. and Zernicka-Goetz, Magdalena (2005) The first cleavage of the mouse zygote predicts the blastocyst axis. Nature, 434 (7031). pp. 391-395. ISSN 0028-0836. doi:10.1038/nature03388. https://resolver.caltech.edu/CaltechAUTHORS:20190419-105737803

[img] Video (QuickTime) (Supplementary Movie S1. Time-lapse image of first cleavage in zygotes expressing H2B-EGFP) - Supplemental Material
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[img] Video (QuickTime) (Supplementary Movie S2. Time-lapse DIC/ fluorescence images of zygotes expressing H2B-EGFP and with marked sperm entry site) - Supplemental Material
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[img] Video (QuickTime) (Supplementary Movie S3. Time-lapse images of first cleavage division in zygotes in which the animal pole was transplanted to 90° of its original position) - Supplemental Material
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[img] Video (QuickTime) (Supplementary Movie S4 section 2. Time-lapse images of a series of control embryos tracking the path of pronuclei migration. Movie in 5 sections) - Supplemental Material
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[img] Video (QuickTime) (Supplementary Movie S4 section 3) - Supplemental Material
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[img] Video (QuickTime) (Supplementary Movie S4 section 4) - Supplemental Material
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[img] Video (QuickTime) (Supplementary Movie S4 section 5) - Supplemental Material
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[img] Video (QuickTime) (Supplementary Movie S4 section 6) - Supplemental Material
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[img] Video (QuickTime) (Supplementary Movie S5 section 2. Time-lapse images of a series of embryos treated for 4 h with 5 g/ml cytochalasin B to depolymerise actin filaments during the time of pronuclei migration. Movie in 6 sections) - Supplemental Material
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[img] Video (QuickTime) (Supplementary Movie S5 section 3) - Supplemental Material
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[img] Video (QuickTime) (Supplementary Movie S5 section 5) - Supplemental Material
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[img] Video (QuickTime) (Supplementary Movie S5 section 7) - Supplemental Material
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[img] MS Word (Supplementary Figure S1. Positions of clones derived from 2-cell blastomeres from experimentally elongated zygotes in relation to blastocyst morphology) - Supplemental Material
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[img] MS Word (Supplementary Figure Legend. Legend to accompany the above Supplementary Figure) - Supplemental Material
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Abstract

One of the unanswered questions in mammalian development is how the embryonic–abembryonic axis of the blastocyst is first established. It is possible that the first cleavage division contributes to this process, because in most mouse embryos the progeny of one two-cell blastomere primarily populate the embryonic part of the blastocyst and the progeny of its sister populate the abembryonic part1,2,3,4. However, it is not known whether the embryonic–abembryonic axis is set up by the first cleavage itself, by polarity in the oocyte that then sets the first cleavage plane with respect to the animal pole, or indeed whether it can be divorced entirely from the first cleavage and established in relation to the animal pole. Here we test the importance of the orientation of the first cleavage by imposing an elongated shape on the zygote so that the division no longer passes close to the animal pole, marked by the second polar body. Non-invasive lineage tracing shows that even when the first cleavage occurs along the short axis imposed by this experimental treatment, the progeny of the resulting two-cell blastomeres tend to populate the respective embryonic and abembryonic parts of the blastocyst. Thus, the first cleavage contributes to breaking the symmetry of the embryo, generating blastomeres with different developmental characteristics.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1038/nature03388DOIArticle
https://rdcu.be/bxRzZPublisherFree ReadCube access
ORCID:
AuthorORCID
Glover, David M.0000-0003-0956-0103
Zernicka-Goetz, Magdalena0000-0002-7004-2471
Additional Information:© 2005 Nature Publishing Group. Received 9 November 2004; accepted 25 January 2005. This work was supported by a Wellcome Trust Senior Research Fellowship to M.Z.-G. and a BBSRC Project Grant to M.Z.-G. and D.M.G. K.P.N. was holding a Marie Curie Fellowship from the European Union. V.E.P. acknowledges support from the NIH. B.P. is on leave from the Department of Experimental Embryology at The Polish Academy of Science, Jastrzebic, Poland. The authors declare that they have no competing financial interests.
Funders:
Funding AgencyGrant Number
Wellcome TrustUNSPECIFIED
Biotechnology and Biological Sciences Research Council (BBSRC)UNSPECIFIED
Marie Curie FellowshipUNSPECIFIED
NIHUNSPECIFIED
Issue or Number:7031
DOI:10.1038/nature03388
Record Number:CaltechAUTHORS:20190419-105737803
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20190419-105737803
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:94818
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:23 Apr 2019 16:30
Last Modified:16 Nov 2021 17:08

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