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Four-cell stage mouse blastomeres have different developmental properties

Piotrowska-Nitsche, Karolina and Perea-Gomez, Aitana and Haraguchi, Seiki and Zernicka-Goetz, Magdalena (2005) Four-cell stage mouse blastomeres have different developmental properties. Development, 132 (3). pp. 479-490. ISSN 0950-1991. doi:10.1242/dev.01602. https://resolver.caltech.edu/CaltechAUTHORS:20190419-105737904

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[img] Video (QuickTime) (Movie 1. Second cleavage divisions in which both two-cell blastomeres are labelled with the beads at their vegetal poles. In the two-cell stage blastomere undertaking an E-division, membrane labelled by the bead does not become displaced) - Supplemental Material
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[img] Video (QuickTime) (Movie 2. Second cleavage divisions in which both two-cell stage blastomeres are labelled with the beads at their vegetal poles) - Supplemental Material
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Abstract

Blastomeres of the early mouse embryo are thought to be equivalent in their developmental properties at least until the eight-cell stage. However, the experiments that have led to this conclusion could not have taken into account either the spatial origin of individual blastomeres or the spatial allocation and fate of their progeny. We have therefore readdressed this issue having defined cell lineages in mouse embryos undergoing different patterns of cleavage in their second division cycle. This has enabled us to identify a major group of embryos in which we can predict not only the spatial origin of each given four-cell blastomeres, but also which region of the blastocyst is most likely to be occupied by its progeny. We show that a pattern of second cleavage divisions in which a meridional division is followed by one that is equatorial or oblique allows us to identify blastomeres that differ in their fate and in their developmental properties both from each other and from their cousins. We find that one of these four-cell stage blastomeres that inherits some vegetal membrane marked in the previous cleavage cycle tends to contribute to mural trophectoderm. The progeny of its sister tend to donate cells to part of the ICM lining the blastocyst cavity and its associated trophectoderm. Chimaeras made entirely of these equatorially or obliquely derived blastomeres show developmental abnormalities in both late preimplantation and early postimplantation development. By contrast, chimaeras made from four-cell stage blastomeres from early meridional divisions develop normally. The developmental defects of chimaeras made from the most vegetal blastomeres that result from later second cleavages are the most severe and following transplantation into foster mothers they fail to develop to term. However, when such individual four-cell blastomeres are surrounded by blastomeres from random positions, they are able to contribute to all embryonic lineages. In conclusion, this study shows that while all four-cell blastomeres can have full developmental potential, they differ in their individual developmental properties according to their origin in the embryo from as early as the four-cell stage.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1242/dev.01602DOIArticle
http://dev.biologists.org/cgi/content/full/132/3/479/DC1PublisherSupporting Information
ORCID:
AuthorORCID
Zernicka-Goetz, Magdalena0000-0002-7004-2471
Additional Information:© 2005 The Company of Biologists. Accepted 29 November 2004. The work was supported by the Wellcome Trust Senior Fellowship to M.Z.G. K.P. is a Marie Curie Fellow. We are grateful to David Glover, Anne McLaren, Jonathon Pines and John Gurdon for their invaluable support and comments on the manuscript. We thank I. Mason for the Fgf8 probe.
Funders:
Funding AgencyGrant Number
Wellcome TrustUNSPECIFIED
Marie Curie FellowshipUNSPECIFIED
Subject Keywords: Cleavage pattern, Cell fate, Chimaeras, Mouse embryo
Issue or Number:3
DOI:10.1242/dev.01602
Record Number:CaltechAUTHORS:20190419-105737904
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20190419-105737904
Official Citation:Four-cell stage mouse blastomeres have different developmental properties Karolina Piotrowska-Nitsche, Aitana Perea-Gomez, Seiki Haraguchi, Magdalena Zernicka-Goetz Development 2005 132: 479-490; doi: 10.1242/dev.01602
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:94819
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:23 Apr 2019 16:27
Last Modified:16 Nov 2021 17:08

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