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Directing pluripotent cell differentiation using "diced RNA" in transient transfection

Carpenter, Lee and Zernicka-Goetz, Magdalena (2004) Directing pluripotent cell differentiation using "diced RNA" in transient transfection. Genesis, 40 (3). pp. 157-163. ISSN 1526-954X. doi:10.1002/gene.20078. https://resolver.caltech.edu/CaltechAUTHORS:20190419-105738149

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Abstract

Embryonic stem (ES) and embryonic carcinoma (EC) cells are pluripotent and have the capacity to differentiate into many cell types. The ability to direct their differentiation should have considerable practical applications. Here, we first report the use of diced short interfering RNAi against Oct4 in a transient approach, to direct differentiation of ES towards the trophectoderm lineage. We then apply this approach to downregulate Smad4 in mouse P19 EC cells. We have found that this leads to an increase in the levels of Pax6 (a neuroectoderm marker), reduction in the levels of Brachyury (a mesoderm marker), and a 3‐fold increase in the number of βIII tubulin‐positive colonies when these cells were allowed to differentiate. This indicates a redirection of cell fate towards the neuroectoderm lineage. Thus, transient RNAi could provide a valuable tool to direct pluripotent cells along specific pathways of differentiation while circumventing permanent genetic changes.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1002/gene.20078DOIArticle
ORCID:
AuthorORCID
Zernicka-Goetz, Magdalena0000-0002-7004-2471
Additional Information:© 2004 Wiley‐Liss. Received 19 April 2004; Accepted 15 July 2004. Contract grant sponsor: Cancer Research UK (CRUK), Contract grant number: C3/A2943. We thank Neil Clarke, Chris Ring, and David Glover for valuable discussions and critical review of this paper. MZG is a Wellcome Trust Senior Research Fellow.
Funders:
Funding AgencyGrant Number
Cancer Research UKC3/A2943
Wellcome TrustUNSPECIFIED
Subject Keywords:RNA interference; ES cells; EC cells; differentiation; Smad4; Oct4
Issue or Number:3
DOI:10.1002/gene.20078
Record Number:CaltechAUTHORS:20190419-105738149
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20190419-105738149
Official Citation:Carpenter, L. and Zernicka‐Goetz, M. (2004), Directing pluripotent cell differentiation using “diced RNA” in transient transfection. Genesis, 40: 157-163. doi:10.1002/gene.20078
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:94821
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:23 Apr 2019 17:22
Last Modified:16 Nov 2021 17:08

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