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A Genome-Wide Study of Gene Activity Reveals Developmental Signaling Pathways in the Preimplantation Mouse Embryo

Wang, Q. Tian and Piotrowska, Karolina and Ciemerych, Maria Anna and Milenkovic, Ljiljana and Scott, Matthew P. and Davis, Ronald W. and Zernicka-Goetz, Magdalena (2004) A Genome-Wide Study of Gene Activity Reveals Developmental Signaling Pathways in the Preimplantation Mouse Embryo. Developmental Cell, 6 (1). pp. 133-144. ISSN 1534-5807. doi:10.1016/s1534-5807(03)00404-0. https://resolver.caltech.edu/CaltechAUTHORS:20190419-105738586

[img] MS Word (Table S1. Number of Oocytes/Embryos in Each Collection) - Supplemental Material
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[img] MS Word (Table S2. Array Data Recapitulated Documented Expression of Known Genes) - Supplemental Material
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[img] MS Word (Table S3. Array Parameters For detailed description of terms, see Schadt et al., 2001, and http://www.dchip.org) - Supplemental Material
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[img] MS Word (Table S4. A Cluster of Maternal Transcripts) - Supplemental Material
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[img] MS Word (Table S5. Oct4 Cofactors and Targets) - Supplemental Material
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[img] MS Word (Table S6. Expression of Signaling Pathway Components) - Supplemental Material
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Abstract

The preimplantation development of the mammalian embryo encompasses a series of critical events: the transition from oocyte to embryo, the first cell divisions, the establishment of cellular contacts, the first lineage differentiation—all the first subtle steps toward a future body plan. Here, we use microarrays to explore gene activity during preimplantation development. We reveal robust and dynamic patterns of stage-specific gene activity that fall into two major phases, one up to the 2-cell stage (oocyte-to-embryo transition) and one after the 4-cell stage (cellular differentiation). The mouse oocyte and early embryo express components of multiple signaling pathways including those downstream of Wnt, BMP, and Notch, indicating that conserved regulators of cell fate and pattern formation are likely to function at the earliest embryonic stages. Overall, these data provide a detailed temporal profile of gene expression that reveals the richness of signaling processes in early mammalian development.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1016/s1534-5807(03)00404-0DOIArticle
ORCID:
AuthorORCID
Zernicka-Goetz, Magdalena0000-0002-7004-2471
Additional Information:© 2004 Cell Press. Under an Elsevier user license. Received 11 November 2003, Revised 17 December 2003, Accepted 17 December 2003, Available online 15 January 2004. This work was supported by NIH grant to R.W.D., and a Wellcome Trust project grant and Senior research fellowship to M.Z.-G. M.P.S. is an Investigator of the Howard Hughes Medical Institute. K.P. is a recipient of Marie Curie fellowship. We thank Randall Moon for β-catenin-GFP construct, Emma Cadwell for help with embryo collections, the Marco Conti lab for help with immunostaining of ovarian sections, David Glover, Karin Lykke-Andersen, Lars Steinmetz, Adam Deutschbauer, Tavvi Neklesa, and Jed Dean for comments, and Wenzhong Xiao for discussions.
Funders:
Funding AgencyGrant Number
NIHUNSPECIFIED
Wellcome TrustUNSPECIFIED
Howard Hughes Medical Institute (HHMI)UNSPECIFIED
Marie Curie FellowshipUNSPECIFIED
Issue or Number:1
DOI:10.1016/s1534-5807(03)00404-0
Record Number:CaltechAUTHORS:20190419-105738586
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20190419-105738586
Official Citation:Q.Tian Wang, Karolina Piotrowska, Maria Anna Ciemerych, Ljiljana Milenkovic, Matthew P. Scott, Ronald W. Davis, Magdalena Zernicka-Goetz, A Genome-Wide Study of Gene Activity Reveals Developmental Signaling Pathways in the Preimplantation Mouse Embryo, Developmental Cell, Volume 6, Issue 1, 2004, Pages 133-144, ISSN 1534-5807, https://doi.org/10.1016/S1534-5807(03)00404-0. (http://www.sciencedirect.com/science/article/pii/S1534580703004040)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:94825
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:23 Apr 2019 17:27
Last Modified:16 Nov 2021 17:08

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