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Developmental fate of embryonic germ cells (EGCs), in vivo and in vitro

Durcova-Hills, Gabriela and Wianny, Florence and Merriman, Julie and Zernicka-Goetz, Magdalena and McLaren, Anne (2003) Developmental fate of embryonic germ cells (EGCs), in vivo and in vitro. Differentiation, 71 (2). pp. 135-141. ISSN 0301-4681. doi:10.1046/j.1432-0436.2003.710204.x.

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Embryonic germ cells (EGCs) derived from mouse primordial germ cells (PGCs) are known both to colonize all cell lineages of the fetus and to make tumors in vivo. When aggregated with eight-cell embryos, EGCs from a new EGC line expressing green fluorescent protein (GFP) were found to contribute preferentially to the epiblast but unexpectedly were also capable of colonizing primary endoderm. When injected under the kidney capsule, EGCs derived from 12.5 days post coitum (dpc) PGCs formed differentiated tumors. The ability of EGCs to differentiate in an organ culture system depends upon their partners in cell culture. When EGCs, marked with a LacZ transgene, were mixed with disaggregated and reaggregated mouse fetal lung in an organ culture system, they remained undifferentiated. In urogenital ridge reaggregates on the other hand, some EGCs were capable of differentiating to form small epithelial cysts.

Item Type:Article
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Zernicka-Goetz, Magdalena0000-0002-7004-2471
Additional Information:© 2003 International Society of Differentiation. Published by Elsevier. Accepted 21 October 2002, Available online 23 November 2009. We are grateful to the Wellcome Trust for financial support (GD-H, MZG, and AM). We are also grateful for financial support to the Cancer Research Campaign (MZG and FW) and the Lister Institute of Preventive Medicine (MZG). We thank Dr. Sheila Barton for her help in both injecting the construct to produce EF1alpha MmGFP transgenic mice and injection of EGCs under the kidney capsule and Dr Takeshi Tada for allowing us to use his LacZ-expressing EGCs.
Funding AgencyGrant Number
Cancer Research CampaignUNSPECIFIED
Lister Institute of Preventive MedicineUNSPECIFIED
Subject Keywords:primordial germ cells; embryonic germ cells; chimera; green fluorescence protein; aggregates; differentiation
Issue or Number:2
Record Number:CaltechAUTHORS:20190419-105935323
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Official Citation:Gabriela Durcova-Hills, Florence Wianny, Julie Merriman, Magdalena Zernicka-Goetz, Anne McLaren, Developmental fate of embryonic germ cells (EGCs), in vivo and in vitro, Differentiation, Volume 71, Issue 2, 2003, Pages 135-141, ISSN 0301-4681, (
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:94828
Deposited By: George Porter
Deposited On:23 Apr 2019 18:07
Last Modified:16 Nov 2021 17:08

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