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Design and Characterization of RNA Nanotubes

Stewart, Jaimie Marie and Geary, Cody and Franco, Elisa (2019) Design and Characterization of RNA Nanotubes. ACS Nano, 13 (5). pp. 5214-5221. ISSN 1936-0851. https://resolver.caltech.edu/CaltechAUTHORS:20190422-091140594

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Abstract

RNA is a functionally rich and diverse biomaterial responsible for regulating several cellular processes. This functionality has been harnessed to build predominately small nanoscale structures for drug delivery and the treatment of disease. The understanding of design principles to build large RNA structures will allow for further control of stoichiometry and spatial arrangement drugs and ligands. We present the design and characterization of RNA nanotubes that self-assemble from programmable monomers, or tiles, formed by five distinct RNA strands. Tiles include double crossover junctions and assemble via single-stranded sticky-end domains. We find that nanotube formation is dependent on the intertile crossover distance. The average length observed for the annealed RNA nanotubes is ≈1.5 μm, with many nanotubes exceeding 10 μm, enabling the characterization of RNA nanotubes length distribution via fluorescence microscopy. Assembled tubes were observed to be stable for more than 24 h, however post-annealing growth under isothermal conditions does not occur. Nanotubes assemble also from RNA tiles modified to include a single-stranded overhang (toehold), suggesting that it may be possible to decorate these large RNA scaffolds with nanoparticles or other nucleic acid molecules.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1021/acsnano.8b09421DOIArticle
https://pubs.acs.org/doi/suppl/10.1021/acsnano.8b09421PublisherSupporting Information
ORCID:
AuthorORCID
Geary, Cody0000-0003-2083-4259
Franco, Elisa0000-0003-1103-2668
Additional Information:© 2019 American Chemical Society. Received 12 December 2018. Date accepted 9 April 2019. Published online 22 April 2019. The authors thank Paul W. K. Rothemund for useful discussions and advise. This work was supported by a U.S. National Science Foundation CAREER grant to E.F. [DMR- 1450747]. The authors declare no competing financial interest.
Funders:
Funding AgencyGrant Number
NSFDMR-1450747
Subject Keywords:RNA, nanotechnology, nucleic acids, self-assembly, nanotubes
Issue or Number:5
Record Number:CaltechAUTHORS:20190422-091140594
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20190422-091140594
Official Citation:Design and Characterization of RNA Nanotubes. Jaimie Marie Stewart, Cody Geary, and Elisa Franco. ACS Nano 2019 13 (5), 5214-5221. DOI: 10.1021/acsnano.8b09421
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:94832
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:22 Apr 2019 23:14
Last Modified:03 Oct 2019 21:07

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