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Site of the previous meiotic division defines cleavage orientation in the mouse embryo

Plusa, Berenika and Grabarek, Joanna B. and Piotrowska, Karolina and Glover, David M. and Zernicka-Goetz, Magdalena (2002) Site of the previous meiotic division defines cleavage orientation in the mouse embryo. Nature Cell Biology, 4 (10). pp. 811-815. ISSN 1465-7392. https://resolver.caltech.edu/CaltechAUTHORS:20190422-100408799

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Abstract

The conservation of early cleavage patterns in organisms as diverse as echinoderms and mammals suggests that even in highly regulative embryos such as the mouse, division patterns might be important for development. Indeed, the first cleavage divides the fertilized mouse egg into two cells: one cell that contributes predominantly to the embryonic part of the blastocyst, and one that contributes to the abembryonic part. Here we show, by removing, transplanting or duplicating the animal or vegetal poles of the mouse egg, that a spatial cue at the animal pole orients the plane of this initial division. Embryos with duplicated animal, but not vegetal, poles show abnormalities in chromosome segregation that compromise their development. Our results show that localized factors in the mammalian egg orient the spindle and so define the initial cleavage plane. In increased dosage, however, these factors are detrimental to the correct execution of division.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1038/ncb860DOIArticle
https://rdcu.be/bygp4PublisherFree ReadCube access
https://doi.org/10.1038/ncb876DOIErratum
https://rdcu.be/bygsiPublisherFree ReadCube access - Erratum
ORCID:
AuthorORCID
Glover, David M.0000-0003-0956-0103
Zernicka-Goetz, Magdalena0000-0002-7004-2471
Additional Information:© 2002 Nature Publishing Group. RECEIVED 9 APRIL 2002, REVISED 10 JUNE 2002, ACCEPTED 31 JULY 2002, PUBLISHED 23 SEPTEMBER 2002. We thank L. Clayton for some of the earlier immunostaining of mouse embryos, and S. Frankenberg, M. Savoian, J. Pines, A. Brand and J. Ahringer for discussions. This work was supported by the Wellcome Trust (M.Z.-G.), Cancer Research UK (D.M.G.) and the Biotechnology and Biological Science Research Council (M.Z.-G. and D.M.G.). The authors declare no competing financial interests. An Erratum to this article was published on 01 November 2002.
Errata:In Plusa et al. (Nature Cell Biology 4, 811–815, (2002)), the incorrect abstract was published. This has now been corrected online and is reproduced below: The conservation of early cleavage patterns in organisms as diverse as echinoderms and mammals suggests that even in highly regulative embryos such as the mouse, division patterns might be important for development1–4. Indeed, the first cleavage divides the fertilized mouse egg into two cells: one cell that contributes predominantly to the embryonic part of the blastocyst, and one that contributes to the abembryonic part5,6. Here we show, by removing, transplanting or duplicating the animal or vegetal poles of the mouse egg, that a spatial cue at the animal pole orients the plane of this initial division. Embryos with duplicated animal, but not vegetal, poles show abnormalities in chromosome segregation that compromise their development. Our results show that localized factors in the mammalian egg orient the spindle and so define the initial cleavage plane. In increased dosage, however, these factors are detrimental to the correct execution of division.
Funders:
Funding AgencyGrant Number
Wellcome TrustUNSPECIFIED
Cancer Research UKUNSPECIFIED
Biotechnology and Biological Sciences Research Council (BBSRC)UNSPECIFIED
Issue or Number:10
Record Number:CaltechAUTHORS:20190422-100408799
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20190422-100408799
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:94838
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:23 Apr 2019 15:53
Last Modified:03 Oct 2019 21:07

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