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Efficient delivery of dsRNA into zona-enclosed mouse oocytes and preimplantation embryos by electroporation

Grabarek, Joanna B. and Plusa, Berenika and Glover, David M. and Zernicka-Goetz, Magdalena (2002) Efficient delivery of dsRNA into zona-enclosed mouse oocytes and preimplantation embryos by electroporation. Genesis, 32 (4). pp. 269-276. ISSN 1526-954X. doi:10.1002/gene.10076. https://resolver.caltech.edu/CaltechAUTHORS:20190422-100408907

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Abstract

Conditions for the electroporation of mouse oocytes and preimplantation embryos have been optimised by following the incorporation of rhodamine labeled dextran. This procedure includes a step to weaken but not remove the zona pellucida that helps achieve good survival. This approach has been applied to introduce double‐stranded RNA for c‐mos into oocytes and green fluorescent protein (GFP) into transgenic GFP‐expressing embryos at the 1‐ and 4‐cell stages. In both cases we were able to observe sequence‐specific interference with the expression of the target gene—a failure of oocytes to arrest at metaphase II and a loss in the green fluorescence of embryos by the morula or blastocyst stages. These effects could be observed in multiple oocytes or embryos allowed to develop together following electroporation.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1002/gene.10076DOIArticle
ORCID:
AuthorORCID
Glover, David M.0000-0003-0956-0103
Zernicka-Goetz, Magdalena0000-0002-7004-2471
Additional Information:© 2002 Wiley‐Liss. Received 12 November 2001; Accepted 18 January 2002. We are grateful to CRC Technology for their support of this work. MZG is a fellow of the Lister Institute of Preventive Medicine.
Funders:
Funding AgencyGrant Number
Cancer Research Campaign TechnologyUNSPECIFIED
Lister Institute of Preventive MedicineUNSPECIFIED
Subject Keywords:mouse oocytes; preimplantation embryos; RNA interference; electroporation
Issue or Number:4
DOI:10.1002/gene.10076
Record Number:CaltechAUTHORS:20190422-100408907
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20190422-100408907
Official Citation:Grabarek, J. B., Plusa, B. , Glover, D. M. and Zernicka‐Goetz, M. (2002), Efficient delivery of dsRNA into zona‐enclosed mouse oocytes and preimplantation embryos by electroporation. Genesis, 32: 269-276. doi:10.1002/gene.10076
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:94839
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:23 Apr 2019 15:43
Last Modified:16 Nov 2021 17:08

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