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Sperm entry position provides a surface marker for the first cleavage plane of the mouse zygote

Plusa, Berenika and Piotrowska, Karolina and Zernicka-Goetz, Magdalena (2002) Sperm entry position provides a surface marker for the first cleavage plane of the mouse zygote. Genesis, 32 (3). pp. 193-198. ISSN 1526-954X. doi:10.1002/gene.10027. https://resolver.caltech.edu/CaltechAUTHORS:20190422-100408995

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Abstract

The sperm entry position (SEP) of the mouse egg, labelled by placing a bead at the fertilisation cone, tends to be associated with the first cleavage plane (Piotrowska and Zernicka‐Goetz: Nature 409:517–521, 2001). Nevertheless, in up to one‐fourth of embryos the cleavage furrow did not pass close to the bead, and following the division the bead marked the cleavage plane in only 60% of cases. This raised the question of whether such variability arose from the labelling itself or had a biological basis. The zona pellucida was not responsible for this effect because similar results were obtained in its presence or absence. However, this variability could be attributable to the large size of the fertilisation cone relative to the SEP. Therefore, we have developed a means of fluorescently labelling sperm that can record the exact site of its penetration when the label transfers to the egg surface. This approach indicates that the SEP marks the first cleavage in the great majority (88%) of embryos. In conclusion, direct sperm labelling shows precisely the correlation between the SEP and the first cleavage, although there is natural variability in this process.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1002/gene.10027DOIArticle
ORCID:
AuthorORCID
Zernicka-Goetz, Magdalena0000-0002-7004-2471
Additional Information:© 2002 Wiley‐Liss. Received 17 September 2001; Accepted 7 December 2001. This work was supported by a Fellowship from the Lister Institute of Preventive Medicine and Wellcome Trust Project Grant to MZG and by a grant from Cancer Research Campaign Technology to MZG and David Glover.
Funders:
Funding AgencyGrant Number
Lister Institute of Preventive MedicineUNSPECIFIED
Wellcome TrustUNSPECIFIED
Cancer Research Campaign TechnologyUNSPECIFIED
Subject Keywords:mouse; sperm; egg; cleavage; polarity
Issue or Number:3
DOI:10.1002/gene.10027
Record Number:CaltechAUTHORS:20190422-100408995
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20190422-100408995
Official Citation:Plusa, B. , Piotrowska, K. and Zernicka‐Goetz, M. (2002), Sperm entry position provides a surface marker for the first cleavage plane of the mouse zygote. Genesis, 32: 193-198. doi:10.1002/gene.10027
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:94840
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:23 Apr 2019 15:41
Last Modified:16 Nov 2021 17:08

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