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Blastomeres arising from the first cleavage division have distinguishable fates in normal mouse development

Piotrowska, Karolina and Wianny, Florence and Pedersen, Roger A. and Zernicka-Goetz, Magdalena (2001) Blastomeres arising from the first cleavage division have distinguishable fates in normal mouse development. Development, 128 (19). pp. 3739-3748. ISSN 0950-1991. https://resolver.caltech.edu/CaltechAUTHORS:20190422-100452455

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Abstract

Two independent studies have recently suggested similar models in which the embryonic and abembryonic parts of the mouse blastocyst become separated already by the first cleavage division. However, no lineage tracing studies carried out so far on early embryos provide the support for such a hypothesis. Thus, to re-examine the fate of blastomeres of the two-cell mouse embryo, we have undertaken lineage tracing studies using a non-perturbing method. We show that two-cell stage blastomeres have a strong tendency to develop into cells that comprise either the embryonic or the abembryonic parts of the blastocyst. Moreover, the two-cell stage blastomere that is first to divide will preferentially contribute its progeny to the embryonic part. Nevertheless, we find that the blastocyst embryonic-abembryonic axis is not perfectly orthogonal to the first cleavage plane, but often shows some angular displacement from it. Consequently, there is a boundary zone adjacent to the interior margin of the blastocoel that is populated by cells derived from both earlier and later dividing blastomeres. The majority of cells that inhabit this boundary region are, however, derived from the later dividing two-cell stage blastomere that contributes predominantly to the abembryonic part of the blastocyst. Thus, at the two-cell stage it is already possible to predict which cell will contribute a greater proportion of its progeny to the abembryonic part of the blastocyst (region including the blastocyst cavity) and which to the embryonic part (region containing the inner cell mass) that will give rise to the embryo proper.


Item Type:Article
Related URLs:
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http://dev.biologists.org/content/128/19/3739.longPublisherArticle
ORCID:
AuthorORCID
Zernicka-Goetz, Magdalena0000-0002-7004-2471
Additional Information:© 2001 The Company of Biologists. Accepted July 11, 2001. We acknowledge Chris Graham for his insightful suggestion of the need for careful lineage tracing in the preimplantation mouse embryo. We are grateful to David Glover for all his great help in preparation of this manuscript and discussions, to Nick Brown for his suggestion of non-invasive methods for cell labelling and to Peter Lawrence for the very helpful suggestion of dissociating blastocysts with trypsin to enable us to record the total number of cells per clone. This work was supported by grants from the Wellcome Trust and Human Frontier Science Program. M. Z.-G. is a Senior Research Fellow of the Lister Foundation for Preventive Medicine and a Stanley Elmore Fellow of Sidney Sussex College.
Funders:
Funding AgencyGrant Number
Wellcome TrustUNSPECIFIED
Human Frontier Science ProgramUNSPECIFIED
Lister Institute for Preventive MedicineUNSPECIFIED
Sidney Sussex CollegeUNSPECIFIED
Subject Keywords:Mouse embryo, Polarity, Blastocyst, Axis, Embryonic, Abembryonic, Body plan
Issue or Number:19
Record Number:CaltechAUTHORS:20190422-100452455
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20190422-100452455
Official Citation:Blastomeres arising from the first cleavage division have distinguishable fates in normal mouse development Karolina Piotrowska, Florence Wianny, Roger A. Pedersen, Magdalena Zernicka-Goetz Development 2001 128: 3739-3748
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:94842
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:23 Apr 2019 15:32
Last Modified:03 Oct 2019 21:07

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