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Animal and vegetal poles of the mouse egg predict the polarity of the embryonic axis, yet are nonessential for development

Ciemerych, Maria Anna and Mesnard, Daniel and Zernicka-Goetz, Magdalena (2000) Animal and vegetal poles of the mouse egg predict the polarity of the embryonic axis, yet are nonessential for development. Development, 127 (16). pp. 3467-3474. ISSN 0950-1991. https://resolver.caltech.edu/CaltechAUTHORS:20190422-101707437

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Abstract

Recent studies suggest early (preimplantation) events might be important in the development of polarity in mammalian embryos. We report here lineage tracing experiments with green fluorescent protein showing that cells located either near to or opposite the polar body at the 8-cell stage of the mouse embryo retain their same relative positions in the blastocyst. Thus they come to lie on either end of an axis of symmetry of the blastocyst that has recently been shown to correlate with the anterior-posterior axis of the postimplantation embryo (see R. J. Weber, R. A. Pedersen, F. Wianny, M. J. Evans and M. Zernicka-Goetz (1999). Development 126, 5591-5598). The embryonic axes of the mouse can therefore be related to the position of the polar body at the 8-cell stage, and by implication, to the animal-vegetal axis of the zygote. However, we also show that chimeric embryos constructed from 2-cell stage blastomeres from which the animal or the vegetal poles have been removed can develop into normal blastocysts and become fertile adult mice. This is also true of chimeras composed of animal or vegetal pole cells derived through normal cleavage to the 8-cell stage. We discuss that although polarity of the postimplantation embryo can be traced back to the 8-cell stage and in turn to the organisation of the egg, it is not absolutely fixed by this time.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dev.biologists.org/content/127/16/3467.longPublisherArticle
ORCID:
AuthorORCID
Zernicka-Goetz, Magdalena0000-0002-7004-2471
Additional Information:© 2000 The Company of Biologists. Accepted 6 June; published on WWW 20 July 2000. We are grateful to our colleagues for support and helpful discussions throughout the course of this work. Lister Institute of Preventive Medicine Fellowship, Wellcome Trust Project Grant and Royal Society Equipment Grant to M. Z.-G. supported this work. We also thank the anonymous reviewers of this paper for their very valuable suggestions.
Funders:
Funding AgencyGrant Number
Lister Institute of Preventive MediicneUNSPECIFIED
Wellcome TrustUNSPECIFIED
Royal SocietyUNSPECIFIED
Subject Keywords:Mouse embryo, Polarity, Animal-vegetal axis, Egg, Blastocyst, Cell lineage, GFP
Issue or Number:16
Record Number:CaltechAUTHORS:20190422-101707437
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20190422-101707437
Official Citation:Animal and vegetal poles of the mouse egg predict the polarity of the embryonic axis, yet are nonessential for development M.A. Ciemerych, D. Mesnard, M. Zernicka-Goetz Development 2000 127: 3467-3474
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:94843
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:23 Apr 2019 15:15
Last Modified:03 Oct 2019 21:07

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