A Caltech Library Service

Following cell fate in the living mouse embryo

Zernicka-Goetz, M. and Pines, J. and McLean Hunter, S. and Dixon, J. P. C. and Siemering, K. R. and Haseloff, J. and Evans, M. J. (1997) Following cell fate in the living mouse embryo. Development, 124 (6). pp. 1133-1137. ISSN 0950-1991.

[img] PDF - Published Version
See Usage Policy.


Use this Persistent URL to link to this item:


It has been difficult to follow many of the dramatic changes in cell fate and cell migration during mouse development. This is because there has been no enduring marker that would allow cells to be recognised in the living embryo. We believe that we have overcome this problem by developing a novel form of green fluorescent protein, named MmGFP, that proves to be easily visible and non toxic to mouse cells and does not perturb embryogenesis. We show that synthetic mRNA encoding MmGFP can be injected into blastomeres to follow the fate of their progeny during preimplantation development. We have made a stable embryonic stem cell line that expresses MmGFP and introduced these fluorescent cells into mouse embryos. For the first time, we have been able to follow the fate of embryonic stem cells in living embryos and to observe directly the contribution of these cells to distinct lineages of the postimplantation embryo. This approach should lead to a more complete description of the dynamics of cell fate in the mouse.

Item Type:Article
Related URLs:
URLURL TypeDescription
Zernicka-Goetz, M.0000-0002-7004-2471
Pines, J.0000-0002-5227-6004
Additional Information:© 1997 The Company of Biologists. (Accepted 20 January 1997) We thank Brendan Cormack for communicating results prior to publication, Hartmund Land for the cdc2 promoter and numerous colleagues for helpful discussions. M. Z.-G. thanks the EMBO for a long-term postdoctoral fellowship. This work was supported by funds from the Wellcome Trust to M. J. E., the MRC to J. H. and the Cancer Research Campaign to J. P.
Funding AgencyGrant Number
European Molecular Biology Organization (EMBO)UNSPECIFIED
Cancer Research CampaignUNSPECIFIED
Subject Keywords:green fluorescent protein, GFP, cell fate, mouse development
Issue or Number:6
Record Number:CaltechAUTHORS:20190422-101710597
Persistent URL:
Official Citation:Following cell fate in the living mouse embryo M. Zernicka-Goetz, J. Pines, S. Hunter McLean, J.P. Dixon, K.R. Siemering, J. Haseloff, M.J. Evans Development 1997 124: 1133-1137
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:94846
Deposited By: George Porter
Deposited On:23 Apr 2019 15:02
Last Modified:03 Oct 2019 21:07

Repository Staff Only: item control page