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Use of Green Fluorescent Protein in Mouse Embryos

Zernicka-Goetz, Magdalena and Pines, Jonathon (2001) Use of Green Fluorescent Protein in Mouse Embryos. Methods, 24 (1). pp. 55-60. ISSN 1046-2023. doi:10.1006/meth.2001.1157.

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Green Fluorescent Protein (GFP) has rapidly been established as a versatile and powerful cell marker in many organisms. Initial problems in using it in mammalian cells were solved by introducing mutations to increase its solubility at higher temperatures, such that GFP has now been used as a reporter in both gene expression and cell lineage studies, and to localize proteins within mammalian cells. GFP has two unique advantages: (i) the protein becomes fluorescent in an autocatalytic reaction, so that it can be introduced into any cell type simply as a cDNA or mRNA, or as protein; (ii) it is “bright” enough to be visualized in living cells under conditions that do not cause photodamage to the cells. In this article we outline the ways in which we have used GFP mRNA and cDNA in our studies of mouse cell lineages, and to characterize the behavior of proteins within the embryos.

Item Type:Article
Related URLs:
URLURL TypeDescription
Zernicka-Goetz, Magdalena0000-0002-7004-2471
Pines, Jonathon0000-0002-5227-6004
Additional Information:© 2001 Academic Press.
Subject Keywords:green fluorescent protein; cell marker; cell lineage; fluorescence; mouse; embryo; development; RNA
Issue or Number:1
Record Number:CaltechAUTHORS:20190422-101735870
Persistent URL:
Official Citation:Magdalena Zernicka-Goetz, Jonathon Pines, Use of Green Fluorescent Protein in Mouse Embryos, Methods, Volume 24, Issue 1, 2001, Pages 55-60, ISSN 1046-2023, (
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:94851
Deposited By: George Porter
Deposited On:23 Apr 2019 15:27
Last Modified:16 Nov 2021 17:08

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