CaltechAUTHORS
  A Caltech Library Service

Progression of mouse oocytes from metaphase I to metaphase II is inhibited by fusion with G2 cells

Grabarek, Joanna and Zernicka-Goetz, Magdalena (2000) Progression of mouse oocytes from metaphase I to metaphase II is inhibited by fusion with G2 cells. Zygote, 8 (2). pp. 145-151. ISSN 0967-1994. https://resolver.caltech.edu/CaltechAUTHORS:20190422-101736181

Full text is not posted in this repository. Consult Related URLs below.

Use this Persistent URL to link to this item: https://resolver.caltech.edu/CaltechAUTHORS:20190422-101736181

Abstract

We show that in contrast to metaphase II oocytes, metaphase I oocytes cannot be activated by fusion with the zygote. Fusion of metaphase I oocytes with G2 zygotes was followed by premature chromosome condensation, with 60% of the hybrids becoming arrested at metaphase I, the remainder progressing and arresting at metaphase II. Hybrids of metaphase I oocytes and M-phase zygotes underwent accelerated maturation, but all arrested at metaphase II. In both cases the arrest could be overcome by treatment with the parthenogenetic activators ethanol and cycloheximide. We discuss these findings in relation to the possibility that the metaphase I oocyte contains cytostatic factor activity that is activated by its zygotic partner. Alternatively, the G2 zygote may provide an inhibitor of anaphase, normally never present in the metaphase I oocyte and which is absent from the M-phase zygote.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1017/s0967199400000927DOIArticle
ORCID:
AuthorORCID
Zernicka-Goetz, Magdalena0000-0002-7004-2471
Additional Information:© 2000 Cambridge University Press. Date submitted: 25.11.99. Date accepted: 20.12.99. We are very grateful to Andrzej K. Tarkowski for support during the course of this work, for carefully reading this manuscript and for many useful comments. We thank Anna M. Ciemerych and David Glover for discussions and for all their help in writing the manuscript.
Subject Keywords: Cell cycle, CSF, Meiotic maturation, Mouse, MPF
Issue or Number:2
Record Number:CaltechAUTHORS:20190422-101736181
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20190422-101736181
Official Citation:Grabarek, J., & Zernicka-Goetz, M. (2000). Progression of mouse oocytes from metaphase I to metaphase II is inhibited by fusion with G2 cells. Zygote, 8(2), 145-151.
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:94854
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:23 Apr 2019 15:12
Last Modified:03 Oct 2019 21:07

Repository Staff Only: item control page