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Cell Lineage Analysis: Applications of Green Fluorescent Protein

Zernicka-Goetz, Magdalena and Pines, Jonathon (2003) Cell Lineage Analysis: Applications of Green Fluorescent Protein. In: Developmental Biology Protocols. Methods in Molecular Biology. Vol.I. No.135. Humana Press , Totowa, NJ, pp. 279-287. ISBN 1592596851.

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The ideal cell lineage marker is one that can be visualized in living tissues without perturbing development. Exogenously applied dyes are very useful, but in many instances there would be advantages to an endogenously expressed marker. Green fluorescent protein (GFP) appears to have the potential to be ideal for this purpose, and with its advent, lineage analysis is entering a new phase in which cells can now be followed in real time in living embryos. GFP is a 27-kDa protein found in the jellyfish Aequorea victoria that absorbs blue light and emits green light. It has the valuable property that the formation of the chromophore is an autocatalytic event that requires no cofactors. Furthermore, the cDNA that encodes GFP has been cloned, and thus GFP can be expressed and will become fluorescent in any organism or cell type. In this chapter, we describe the properties of GFP and the different mutants available to study cell lineages in different organisms. We will mainly concentrate on their application to the analysis of cell lineages in frogs and mice.

Item Type:Book Section
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URLURL TypeDescription
Zernicka-Goetz, Magdalena0000-0002-7004-2471
Pines, Jonathon0000-0002-5227-6004
Additional Information:© Humana Press Inc. 2000.
Subject Keywords:Green Fluorescent Protein; Codon Usage; Yellow Fluorescent Protein; Green Fluorescent Protein Fluorescence; Green Fluorescent Protein Signal
Series Name:Methods in Molecular Biology
Issue or Number:135
Record Number:CaltechAUTHORS:20190422-101736360
Persistent URL:
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:94856
Deposited By: George Porter
Deposited On:23 Apr 2019 15:08
Last Modified:16 Nov 2021 17:08

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