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Spontaneous and induced activation of rat oocytes

Zernicka-Goetz, Magdalena (1991) Spontaneous and induced activation of rat oocytes. Molecular Reproduction and Development, 28 (2). pp. 169-176. ISSN 1040-452X. https://resolver.caltech.edu/CaltechAUTHORS:20190422-101736808

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Abstract

Ovulated rat oocytes undergo spontaneous activation during in vitro culture. After extrusion of the second polar body, they do not enter interphase but are arrested again in next metaphase‐like stage (M III arrest). The present study demonstrates that puromycin and chloral hydrate can trigger transition to interphase of metaphase II and spontaneously (incompletely) activated rat oocytes. The response of oocytes to these activators depends on their stage at the time of application of a stimulus. Metaphase II oocytes enter interphase at 86.8% when treated with puromycin and in 28.7% after chloral hydrate activation. Oocytes activated with chloral hydrate at the time of spontaneously induced anaphase II enter interphase at 64.8%, but after reaching the stage of telophase II their capability to shift to interphase is again low (28.8%). Finally, M III oocytes cannot be forced to enter interphase by either chloral hydrate or puromycin treatment. This study shows that resumption of the second meiotic division and transition to interphase–‐the two processes that normally occur in succession as a response to oocyte activatin‐–can be experimentally separated.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1002/mrd.1080280210DOIArticle
ORCID:
AuthorORCID
Zernicka-Goetz, Magdalena0000-0002-7004-2471
Additional Information:© 1991 Wiley‐Liss. Manuscript received: 03 May 1990. Manuscript accepted: 19 September 1990.
Subject Keywords:Parthenogenesis; Puromycin; Chloral hydrate; Handling procedures
Issue or Number:2
Record Number:CaltechAUTHORS:20190422-101736808
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20190422-101736808
Official Citation:Zernicka‐Goetz, M. (1991), Spontaneous and induced activation of rat oocytes. Mol. Reprod. Dev., 28: 169-176. doi:10.1002/mrd.1080280210
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:94861
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:23 Apr 2019 20:02
Last Modified:03 Oct 2019 21:07

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