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Achromatization method for multichannel fluorescence imaging systems

Pawlowski, Michal E. and Yang, Yiran and Wong, Cynthia and Tkaczyk, Tomasz S. (2019) Achromatization method for multichannel fluorescence imaging systems. Optical Engineering, 58 (1). Art. No. 015106. ISSN 0091-3286. https://resolver.caltech.edu/CaltechAUTHORS:20190503-140319875

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Abstract

An achromatization method optimized for dual-channel imaging is developed. Dichroic mirrors are employed to split and recombine narrowband signals, and separation between catoptric components is used to minimize the longitudinal chromatic shift. An achromatic system based on this principle could be built from singlet lenses, since refractive element properties such as dispersion and power are not utilized to optimize wavelength-dependent performance. To demonstrate the validity of the proposed solution, a prototype miniature fluorescence microscope optimized for two emission lines of acridine orange (525 and 650 nm) is built. To reduce the cost and accelerate assembly, the system is built from commercially available optical components. The optical train consisted of two plastic singlet lenses combined with a pair of dichroic mirrors. Optical performance of the prototype is evaluated by imaging a bar line target at both design wavelengths. To demonstrate the potential of the proposed design strategy, the achromatic system prototype is used to measure a two-part white blood cells differential count on a venous blood sample. Data from the prototype fluorescence microscope are compared against results from a commercially available blood analyzer, and the difference between both instruments is within 20%.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1117/1.oe.58.1.015106DOIArticle
ORCID:
AuthorORCID
Yang, Yiran0000-0001-8770-8746
Additional Information:© 2019 Society of Photo-Optical Instrumentation Engineers (SPIE). Paper 181485 received Oct. 16, 2018; accepted for publication Dec. 28, 2018; published online Jan. 22, 2019. This work was financially supported by generous grants provided by NIH R21EB016832 and R01CA186132. We would like to thank Catherine Majors from Rebecca Richards-Kortum’s laboratory for preparing initial blood samples. Additionally, we would like to thank Jason Dwight for fruitful discussions and help in the preparation of this paper. Authors do not have relevant financial interests in this paper. Tomasz S. Tkaczyk has financial interests in Attoris LLC focusing on hyperspectral technologies.
Funders:
Funding AgencyGrant Number
NIHR21EB016832
NIHR01CA186132
Subject Keywords:fluorescence microscopy; optical design and fabrication; medical optics and biotechnology; diagnostic instrumentation
Issue or Number:1
Record Number:CaltechAUTHORS:20190503-140319875
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20190503-140319875
Official Citation:Michal E. Pawlowski, Yiran Yang, Cynthia Wong, and Tomasz S. Tkaczyk "Achromatization method for multichannel fluorescence imaging systems," Optical Engineering 58(1), 015106 (22 January 2019). https://doi.org/10.1117/1.OE.58.1.015106
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:95212
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:03 May 2019 21:14
Last Modified:26 Nov 2019 00:35

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