CaltechAUTHORS
  A Caltech Library Service

Multiplexed-CREATE Selection Yields AAV Vectors Targeting Different Cell Types of the Central Nervous System Following Systemic Delivery

Ravindra Kumar, Sripriya and Chen, Xinhong and Deverman, Benjamin E. and Brown, David and Dobreva, Tatyana and Huang, Qin and Ding, Xiaozhe and Luo, Yicheng and Einarsson, Petur H. and Goeden, Nick and Flytzanis, Nicholas and Greenbaum, Alon and Gradinaru, Viviana (2019) Multiplexed-CREATE Selection Yields AAV Vectors Targeting Different Cell Types of the Central Nervous System Following Systemic Delivery. Molecular Therapy, 27 (4). Art. No. 99. ISSN 1525-0016. https://resolver.caltech.edu/CaltechAUTHORS:20190503-152305499

Full text is not posted in this repository. Consult Related URLs below.

Use this Persistent URL to link to this item: https://resolver.caltech.edu/CaltechAUTHORS:20190503-152305499

Abstract

Recombinant adeno-associated viral (rAAV) capsids are widely accepted as safe gene delivery vehicles in research laboratories and in gene therapy clinical trials and there is potential to further improve their usage by evolving the surface of the capsids to enhance their affinity to specific cell-types or tissues after intravenous (IV) delivery. To this end, we built upon our previous Cre recombination-based AAV targeted evolution (CREATE) method (Deverman et al, Nat. Biotech., 2016; Chan et al., Nat. Neurosci., 2017) to develop Multiplexed-CREATE (M-CREATE). M-CREATE facilitates both positive and negative selections and minimizes the propagation of biases from successive rounds of selection via synthetic library generation. In addition to increasing the confidence in the selections, M-CREATE enables a detailed characterization of the selection process, improving our understanding of selection progression and outcome.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1016/j.ymthe.2019.04.004DOIAbstracts
ORCID:
AuthorORCID
Ravindra Kumar, Sripriya0000-0001-6033-7631
Deverman, Benjamin E.0000-0002-6223-9303
Luo, Yicheng0000-0003-3704-2389
Flytzanis, Nicholas0000-0002-7921-9392
Greenbaum, Alon0000-0002-2897-876X
Gradinaru, Viviana0000-0001-5868-348X
Additional Information:© 2019 Elsevier Inc.
Issue or Number:4
Record Number:CaltechAUTHORS:20190503-152305499
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20190503-152305499
Official Citation:ASGCT 22nd Annual Meeting Abstracts, Molecular Therapy, Volume 27, Issue 4, Supplement 1, 2019, Pages 1-465, ISSN 1525-0016, https://doi.org/10.1016/j.ymthe.2019.04.004. (http://www.sciencedirect.com/science/article/pii/S1525001619301698)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:95219
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:03 May 2019 22:50
Last Modified:09 Mar 2020 13:19

Repository Staff Only: item control page