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Implications of Rewiring Bacterial Quorum Sensing

Haseltine, Eric L. and Arnold, Frances H. (2008) Implications of Rewiring Bacterial Quorum Sensing. Applied and Environmental Microbiology, 74 (2). pp. 437-445. ISSN 0099-2240. PMCID PMC2223271. doi:10.1128/AEM.01688-07.

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Bacteria employ quorum sensing, a form of cell-cell communication, to sense changes in population density and regulate gene expression accordingly. This work investigated the rewiring of one quorum-sensing module, the lux circuit from the marine bacterium Vibrio fischeri. Steady-state experiments demonstrate that rewiring the network architecture of this module can yield graded, threshold, and bistable gene expression as predicted by a mathematical model. The experiments also show that the native lux operon is most consistent with a threshold, as opposed to a bistable, response. Each of the rewired networks yielded functional population sensors at biologically relevant conditions, suggesting that this operon is particularly robust. These findings (i) permit prediction of the behaviors of quorum-sensing operons in bacterial pathogens and (ii) facilitate forward engineering of synthetic gene circuits.

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Arnold, Frances H.0000-0002-4027-364X
Additional Information:© 2008, American Society for Microbiology. Received 23 July 2007/ Accepted 13 November 2007. Published ahead of print on 26 November 2007. E.L.H. gratefully acknowledges support from the Caltech Center for Biological Circuit Design and the National Institutes of Health under Ruth L. Kirschstein National Research Service Award 5F32CA120055. F.H.A. acknowledges support from the U.S. NSF and NIH. We thank Ron Weiss, Subhayu Basu, and Ming-Tang Chen for providing genetic constructs and experimental advice. We also thank Cynthia Collins, Katie Brenner, Cara Tracewell, and Michael Dougherty for helpful discussions and experimental advice. Supplemental material for this article may be found at
Funding AgencyGrant Number
Caltech Center for Biological Circuit DesignUNSPECIFIED
NIH Predoctoral Fellowship5F32CA120055
Issue or Number:2
PubMed Central ID:PMC2223271
Record Number:CaltechAUTHORS:HASaem08
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:9522
Deposited By: Archive Administrator
Deposited On:29 Jan 2008
Last Modified:01 Jun 2023 23:31

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