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Mapping of cis-regulatory sites in the promoter of testis-specific Stellate genes of Drosophila melanogaster

Olenkina, O. M. and Egorova, K. S. and Aravin, A. A. and Naumova, N. M. and Gvozdev, V. A. and Olenina, L. V. (2012) Mapping of cis-regulatory sites in the promoter of testis-specific Stellate genes of Drosophila melanogaster. Biochemistry (Moscow), 77 (11). pp. 1285-1293. ISSN 0006-2979. https://resolver.caltech.edu/CaltechAUTHORS:20190508-083305960

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Abstract

Tandem Stellate genes organized into two clusters in heterochromatin and euchromatin of the X-chromosome are part of the Ste-Su(Ste) genetic system required for maintenance of male fertility and reproduction of Drosophila melanogaster. Stellate genes encode a regulatory subunit of protein kinase CK2 and are the main targets of germline-specific piRNA-silencing; their derepression leads to appearance of protein crystals in spermatocytes, meiotic disturbances, and male sterility. A short promoter region of 134 bp appears to be sufficient for testis-specific transcription of Stellate, and it contains three closely located cis-regulatory elements called E-boxes. By using reporter analysis, we confirmed a strong functionality of the E-boxes in the Stellate promoter for in vivo transcription. Using selective mutagenesis, we have shown that the presence of the central E-box 2 is preferable to maintain a high-level testis-specific transcription of the reporter gene under the Stellate promoter. The Stellate promoter provides transcription even in heterochromatin, and corresponding mRNAs are translated with the generation of full-size protein products in case of disturbances in the piRNA-silencing process. We have also shown for the first time that the activity of the Stellate promoter is determined by chromatin context of the X-chromosome in male germinal cells, and it increases at about twofold when relocating in autosomes.


Item Type:Article
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https://doi.org/10.1134/s0006297912110077DOIArticle
https://rdcu.be/bASK4PublisherFree ReadCube access
Additional Information:© 2012 Pleiades Publishing, Ltd. Original Russian Text © O. M. Olenkina, K. S. Egorova, A. A. Aravin, N. M. Naumova, V. A. Gvozdev, L. V. Olenina, 2012, published in Biokhimiya, 2012, Vol. 77, No. 11, pp. 1536-1545. Originally published in Biochemistry (Moscow) On-Line Papers in Press, as Manuscript BM12-191, October 21, 2012. Received June 22, 2012; Revision received July 3, 2012. This study was supported by the fundamental research program of the Presidium of the Russian Academy of Sciences “Molecular and Cell Biology” and by the Russian Foundation for Basic Research (grant Nos. 10-04-00535-a and 11-04-00017-a).
Funders:
Funding AgencyGrant Number
Presidium of the Russian Academy of SciencesUNSPECIFIED
Russian Foundation for Basic Research10-04-00535-a
Russian Foundation for Basic Research11-04-00017-a
Subject Keywords:promoter; spermatogenesis; Drosophila; Stellate genes; heterochromatin
Issue or Number:11
Record Number:CaltechAUTHORS:20190508-083305960
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20190508-083305960
Official Citation:Olenkina, O.M., Egorova, K.S., Aravin, A.A. et al. Biochemistry Moscow (2012) 77: 1285. https://doi.org/10.1134/S0006297912110077
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:95337
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:08 May 2019 15:48
Last Modified:03 Oct 2019 21:12

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