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Small RNA-based silencing strategies for transposons in the process of invading Drosophila species

Rozhkov, Nikolay V. and Aravin, Alexei A. and Zelentsova, Elena S. and Schostak, Natalia G. and Sachidanandam, Ravi and McCombie, W. Richard and Hannon, Gregory J. and Evgen'ev, Michael B. (2010) Small RNA-based silencing strategies for transposons in the process of invading Drosophila species. RNA, 16 (8). pp. 1634-1645. ISSN 1355-8382. PMCID PMC2905761.

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Colonization of a host by an active transposon can increase mutation rates or cause sterility, a phenotype termed hybrid dysgenesis. As an example, intercrosses of certain Drosophila virilis strains can produce dysgenic progeny. The Penelope element is present only in a subset of laboratory strains and has been implicated as a causative agent of the dysgenic phenotype. We have also introduced Penelope into Drosophila melanogaster, which are otherwise naive to the element. We have taken advantage of these natural and experimentally induced colonization processes to probe the evolution of small RNA pathways in response to transposon challenge. In both species, Penelope was predominantly targeted by endo-small-interfering RNAs (siRNAs) rather than by piwi-interacting RNAs (piRNAs). Although we do observe correlations between Penelope transcription and dysgenesis, we could not correlate differences in maternally deposited Penelope piRNAs with the sterility of progeny. Instead, we found that strains that produced dysgenic progeny differed in their production of piRNAs from clusters in subtelomeric regions, possibly indicating that changes in the overall piRNA repertoire underlie dysgenesis. Considered together, our data reveal unexpected plasticity in small RNA pathways in germ cells, both in the character of their responses to invading transposons and in the piRNA clusters that define their ability to respond to mobile elements.

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Additional Information:© 2010 RNA Society. Freely available online through the RNA Open Access option. Received April 8, 2010; accepted May 21, 2010. We thank M. Rooks, Laura Cardone, and Melissa Kramer for help with Illumina sequencing; G. Assaf and O. Tam for help with bioinformatics analysis; and E. Rozhkova for fly husbandry. We also thank members of the Hannon laboratory for helpful discussions. G.J.H. is an investigator of the Howard Hughes Medical institute, and this work was supported by grants from the NIH and by a kind gift from Kathryn W. Davis. Work at the IMB was supported by the Program of Molecular and Cellular Biology RAN. Sequences reported in this study can be accessed using GEO accession number: GSE22067.
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Howard Hughes Medical Institute (HHMI)UNSPECIFIED
Subject Keywords:Drosophila virilis; piRNA; siRNA; transposon
Issue or Number:8
PubMed Central ID:PMC2905761
Record Number:CaltechAUTHORS:20190508-084004819
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:95338
Deposited By: Tony Diaz
Deposited On:08 May 2019 15:49
Last Modified:03 Oct 2019 21:12

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