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Developmentally Regulated piRNA Clusters Implicate MILI in Transposon Control

Aravin, Alexei A. and Sachidanandam, Ravi and Girard, Angelique and Fejes Toth, Katalin and Hannon, Gregory J. (2007) Developmentally Regulated piRNA Clusters Implicate MILI in Transposon Control. Science, 316 (5825). pp. 744-747. ISSN 0036-8075. doi:10.1126/science.1142612.

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Nearly half of the mammalian genome is composed of repeated sequences. In Drosophila, Piwi proteins exert control over transposons. However, mammalian Piwi proteins, MIWI and MILI, partner with Piwi-interacting RNAs (piRNAs) that are depleted of repeat sequences, which raises questions about a role for mammalian Piwi's in transposon control. A search for murine small RNAs that might program Piwi proteins for transposon suppression revealed developmentally regulated piRNA loci, some of which resemble transposon master control loci of Drosophila. We also find evidence of an adaptive amplification loop in which MILI catalyzes the formation of piRNA 5′ ends. Mili mutants derepress LINE-1 (L1) and intracisternal A particle and lose DNA methylation of L1 elements, demonstrating an evolutionarily conserved role for PIWI proteins in transposon suppression.

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Fejes Toth, Katalin0000-0001-6558-2636
Additional Information:© 2007 American Association for the Advancement of Science. Received 16 March 2007; accepted 30 March 2007. Published online 19 April 2007. piRNA sequences are available in the Gene Expression Omnibus (GEO) database (accession # GSE7414). We thank H. Lin (Yale University) for the Mili knockout mouse. A.A.A. is supported by a Cold Spring Harbor Laboratory Association fellowship. A.G. is a Florence Gould Fellow of the Watson School of Biological Sciences. G.J.H. is an HHMI investigator. This work was supported by grants from NIH and from Katherine W. Davis (G.J.H.).
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Cold Spring Harbor LaboratoryUNSPECIFIED
Howard Hughes Medical Institute (HHMI)UNSPECIFIED
Katherine W. DavisUNSPECIFIED
Issue or Number:5825
Record Number:CaltechAUTHORS:20190509-090419753
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Official Citation:Developmentally Regulated piRNA Clusters Implicate MILI in Transposon Control. BY ALEXEI A. ARAVIN, RAVI SACHIDANANDAM, ANGELIQUE GIRARD, KATALIN FEJES-TOTH, GREGORY J. HANNON. SCIENCE 04 MAY 2007 : 744-747; doi: 10.1126/science.1142612
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:95364
Deposited By: Tony Diaz
Deposited On:09 May 2019 17:33
Last Modified:16 Nov 2021 17:12

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