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Discrete Small RNA-Generating Loci as Master Regulators of Transposon Activity in Drosophila

Brennecke, Julius and Aravin, Alexei A. and Stark, Alexander and Dus, Monica and Kellis, Manolis and Sachidanandam, Ravi and Hannon, Gregory J. (2007) Discrete Small RNA-Generating Loci as Master Regulators of Transposon Activity in Drosophila. Cell, 128 (6). pp. 1089-1103. ISSN 0092-8674. https://resolver.caltech.edu/CaltechAUTHORS:20190509-101139052

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Abstract

Drosophila Piwi-family proteins have been implicated in transposon control. Here, we examine piwi-interacting RNAs (piRNAs) associated with each Drosophila Piwi protein and find that Piwi and Aubergine bind RNAs that are predominantly antisense to transposons, whereas Ago3 complexes contain predominantly sense piRNAs. As in mammals, the majority of Drosophila piRNAs are derived from discrete genomic loci. These loci comprise mainly defective transposon sequences, and some have previously been identified as master regulators of transposon activity. Our data suggest that heterochromatic piRNA loci interact with potentially active, euchromatic transposons to form an adaptive system for transposon control. Complementary relationships between sense and antisense piRNA populations suggest an amplification loop wherein each piRNA-directed cleavage event generates the 5′ end of a new piRNA. Thus, sense piRNAs, formed following cleavage of transposon mRNAs may enhance production of antisense piRNAs, complementary to active elements, by directing cleavage of transcripts from master control loci.


Item Type:Article
Related URLs:
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https://doi.org/10.1016/j.cell.2007.01.043DOIArticle
Additional Information:© 2007 Elsevier Inc. Under an Elsevier user license. Received 23 October 2006, Revised 8 December 2006, Accepted 19 January 2007, Available online 8 March 2007. We thank members of the Hannon laboratory for helpful discussions and support. Ahmet Denli (CSHL) provided antibodies, and Stuart Shuman (MSKCC) provided the expression vector for Rnl2. We thank Andrew Olson and Ted Roeder for bioinformatic support and Stephen Hearn (CSHL) for assistance with confocal microscopy. J.B. and A.S. are supported by fellowships from the Ernst Schering Foundation. A.A. is supported by a fellowship from the CSHL Association. M.D. is an Engelhorn Scholar of the Watson School of Biological Sciences. G.J.H. is an investigator of the Howard Hughes Medical Institute. This work was supported by grants from the N.I.H. (G.J.H.). Accession Numbers: The GenBank accession number for Ago3 in this paper is EF211827. The GEO accession numbers for all piRNAs from this study are GSE6734, GSM154618, GSM154620, GSM154621, and GSM154622.
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Funding AgencyGrant Number
Ernst Schering FoundationUNSPECIFIED
Cold Spring Harbor LaboratoryUNSPECIFIED
Howard Hughes Medical Institute (HHMI)UNSPECIFIED
NIHUNSPECIFIED
Issue or Number:6
Record Number:CaltechAUTHORS:20190509-101139052
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20190509-101139052
Official Citation:Julius Brennecke, Alexei A. Aravin, Alexander Stark, Monica Dus, Manolis Kellis, Ravi Sachidanandam, Gregory J. Hannon, Discrete Small RNA-Generating Loci as Master Regulators of Transposon Activity in Drosophila, Cell, Volume 128, Issue 6, 2007, Pages 1089-1103, ISSN 0092-8674, https://doi.org/10.1016/j.cell.2007.01.043. (http://www.sciencedirect.com/science/article/pii/S0092867407002577)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:95370
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:09 May 2019 17:18
Last Modified:03 Oct 2019 21:12

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