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Dissection of a Natural RNA Silencing Process in the Drosophila melanogaster Germ Line

Aravin, Alexei A. and Klenov, Mikhail S. and Vagin, Vasilii V. and Bantignies, Frédéric and Cavalli, Giacomo and Gvozdev, Vladimir A. (2004) Dissection of a Natural RNA Silencing Process in the Drosophila melanogaster Germ Line. Molecular and Cellular Biology, 24 (15). pp. 6742-6750. ISSN 0270-7306. PMCID PMC444866. https://resolver.caltech.edu/CaltechAUTHORS:20190509-134457510

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Abstract

To date, few natural cases of RNA-silencing-mediated regulation have been described. Here, we analyzed repression of testis-expressed Stellate genes by the homologous Suppressors of Stellate [Su(Ste)] repeats that produce sense and antisense short RNAs. The Stellate promoter is dispensable for suppression, but local disturbance of complementarity between the Stellate transcript and the Su(Ste) repeats impairs silencing. Using in situ RNA hybridization, we found temporal control of the expression and spatial distribution of sense and antisense Stellate and Su(Ste) transcripts in germinal cells. Antisense Su(Ste) transcripts accumulate in the nuclei of early spermatocytes before the appearance of sense transcripts. The sense and antisense transcripts are colocalized in the nuclei of mature spermatocytes, placing the initial step of silencing in the nucleus and suggesting formation of double-stranded RNA. Mutations in the aubergine and spindle-E genes, members of the Argonaute and RNA helicase gene families, respectively, impair silencing by eliminating the short Su(Ste) RNA, but have no effect on microRNA production. Thus, different small RNA-containing complexes operate in the male germ line.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1128/mcb.24.15.6742-6750.2004DOIArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC444866PubMed CentralArticle
ORCID:
AuthorORCID
Cavalli, Giacomo0000-0003-3709-3469
Additional Information:© 2004 American Society for Microbiology. Received 12 November 2003; Returned for modification 31 December 2003; Accepted 6 April 2004. We thank Leonard Robbins, Yair Dorsett, and Phillip Zamore for text improvements and critical comments. This work was supported by grants from the Russian Foundation for Basic Researches (N 02-04-48498), INTAS (N 01-0279), the Russian Foundation for Science School Support (N 2074, 2003.4), the Physics-Chemical Biology Program of RAS, and INTAS young scientist fellowship YSF 00-243 to A.A.A. The work of F.B. and G.C. was supported by grants from the CNRS (ATIPE), the Association pour la Recherche sur le Cancer, and the Human Frontier Science Program Organization.
Funders:
Funding AgencyGrant Number
Russian Foundation for Basic Research02-04-48498
Russian Foundation for Basic Research01-0279
Russian Foundation for Science School Support2074, 2003.4
Russian Academy of SciencesUNSPECIFIED
International Association for the Promotion of Cooperation with Scientists from the New Independent StatesYSF 00-243
Centre National de la Recherche Scientifique (CNRS)UNSPECIFIED
Association pour la Recherche sur le CancerUNSPECIFIED
Human Frontier Science ProgramUNSPECIFIED
Issue or Number:15
PubMed Central ID:PMC444866
Record Number:CaltechAUTHORS:20190509-134457510
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20190509-134457510
Official Citation:Dissection of a Natural RNA Silencing Process in the Drosophila melanogaster Germ Line. Alexei A. Aravin, Mikhail S. Klenov, Vasilii V. Vagin, Frédéric Bantignies, Giacomo Cavalli, Vladimir A. Gvozdev. Molecular and Cellular Biology Jul 2004, 24 (15) 6742-6750; DOI: 10.1128/MCB.24.15.6742-6750.2004
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:95379
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:09 May 2019 20:51
Last Modified:03 Oct 2019 21:12

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