Small RNA Profile during Drosophila melanogaster Development

Aravin, Alexei A. and Lagos-Quintana, Mariana and Yalcin, Abdullah and Zavolan, Mihaela and Marks, Debora and Snyder, Ben and Gaasterland, Terry and Meyer, Jutta and Tuschl, Thomas (2003) Small RNA Profile during Drosophila melanogaster Development. Developmental Cell, 5 (2). pp. 337-350. ISSN 1534-5807. doi:10.1016/s1534-5807(03)00228-4. https://resolver.caltech.edu/CaltechAUTHORS:20190509-142555498

	PDF (Table S1. Size Distribution of All Small RNAs Cloned from cDNA Libraries of D. melanogaster) - Supplemental Material See Usage Policy. 51kB
	PDF (Table S2. Nucleotide Distribution for Small RNAs Cloned from the cDNA Libraries of D. melanogaster in Percentage) - Supplemental Material See Usage Policy. 36kB
	PDF (Table S3. miRNA Gene Location and Sequence Conservation) - Supplemental Material See Usage Policy. 26kB
	PDF (Table S4. miRNA Fold-Back Precursors) - Supplemental Material See Usage Policy. 51kB
	PDF (Table S5. Repeat-Associated Small Interfering RNAs from Different D. melanogaster Developmental Stages) - Supplemental Material See Usage Policy. 122kB

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Abstract

Small RNAs ranging in size between 20 and 30 nucleotides are involved in different types of regulation of gene expression including mRNA degradation, translational repression, and chromatin modification. Here we describe the small RNA profile of Drosophila melanogaster as a function of development. We have cloned and sequenced over 4000 small RNAs, 560 of which have the characteristics of RNase III cleavage products. A nonredundant set of 62 miRNAs was identified. We also isolated 178 repeat-associated small interfering RNAs (rasiRNAs), which are cognate to transposable elements, satellite and microsatellite DNA, and Suppressor of Stellate repeats, suggesting that small RNAs participate in defining chromatin structure. rasiRNAs are most abundant in testes and early embryos, where regulation of transposon activity is critical and dramatic changes in heterochromatin structure occur.

Item Type:

Article

Related URLs:

URL	URL Type	Description
https://doi.org/10.1016/s1534-5807(03)00228-4	DOI	Article

Additional Information:

© 2003 Cell Press. Published by Elsevier Inc. Received 16 June 2003, Revised 23 June 2003, Accepted 27 June 2003, Available online 16 April 2004. We thank G. Dowe and M. Killian for sequencing, W. Lendeckel for help on cloning, A. Hüttenhofer for providing D. melanogaster noncoding RNA sequences to facilitate sequence annotation, Y. Dorsett, M. Landthaler, S. Pfeffer, J. Silverman, and J. Martinez for comments on the manuscript, H. Jäckle, R. Rivera-Pomar, and R. Lührmann for support. This work was funded by a Bundesministerium und Forschung (BMBF) Biofuture grant number 0311856, Program Physico-Chemical Biology RAS, and an EMBO short term fellowship ASTF 9963 to A.A.

Funders:

Funding Agency	Grant Number
Bundesministerium für Bildung und Forschung (BMBF)	0311856
Max Planck Institute	UNSPECIFIED
European Molecular Biology Organization (EMBO)	ASTF 9963

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DOI:

10.1016/s1534-5807(03)00228-4

Record Number:

CaltechAUTHORS:20190509-142555498

Persistent URL:

https://resolver.caltech.edu/CaltechAUTHORS:20190509-142555498

Official Citation:

Alexei A. Aravin, Mariana Lagos-Quintana, Abdullah Yalcin, Mihaela Zavolan, Debora Marks, Ben Snyder, Terry Gaasterland, Jutta Meyer, Thomas Tuschl, The Small RNA Profile during Drosophila melanogaster Development, Developmental Cell, Volume 5, Issue 2, 2003, Pages 337-350, ISSN 1534-5807, https://doi.org/10.1016/S1534-5807(03)00228-4. (http://www.sciencedirect.com/science/article/pii/S1534580703002284)

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No commercial reproduction, distribution, display or performance rights in this work are provided.

ID Code:

95380

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CaltechAUTHORS

Deposited By:

Tony Diaz

Deposited On:

09 May 2019 21:34

Last Modified:

16 Nov 2021 17:12

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