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Mapping of a mutation, polB100, affecting deoxyribonucleic acid polymerase II in Escherichia coli K-12

Campbell, Judith L. and Shizuya, Hiroaki and Richardson, Charles C. (1974) Mapping of a mutation, polB100, affecting deoxyribonucleic acid polymerase II in Escherichia coli K-12. Journal of Bacteriology, 119 (2). pp. 494-499. ISSN 0021-9193. PMCID PMC245632. https://resolver.caltech.edu/CaltechAUTHORS:20190510-105514765

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Abstract

Direct assay for deoxyribonucleic acid polymerase II in extracts has been used to screen recombinants for the polB allele in Hfr x F^- crosses, F-ductants in episome transfer, and transductants in generalized transduction by phage P1. The polB gene is located at 2 min on the Escherichia coli linkage map; it is 39 to 64% co-transducible with leu. A mutant, E. coli polA1 polB100 polC (ts), deficient in deoxyribonucleic acid polymerases I and II and having a thermolabile deoxyribonucleic acid polymerase III, has been prepared by the P1-mediated cross: P1 (HMS85 polB100) x E. coli BT1026 polA1 polC (ts) leu^-.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://jb.asm.org/content/119/2/494PublisherArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC245632/PubMed CentralArticle
Additional Information:© 1974 American Society for Microbiology. Received for publication 26 April 1974. This investigation was supported by Public Health Service grant AI-06045 from the National Institute of Allergy and Infectious Diseases, grant NP-1C from the American Cancer Society, Incorporated, and Public Health Service Research Career Development Award GM 13,634 (to C.C.R.) from the National Institute of General Medical Sciences.
Funders:
Funding AgencyGrant Number
NIHAI-06045
American Cancer SocietyNP-1C
NIHGM 13634
Issue or Number:2
PubMed Central ID:PMC245632
Record Number:CaltechAUTHORS:20190510-105514765
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20190510-105514765
Official Citation:Mapping of a Mutation, polB100, Affecting Deoxyribonucleic Acid Polymerase II in Escherichia coli K-12 Judith L. Campbell, Hiroaki Shizuya, Charles C. Richardson Journal of Bacteriology Aug 1974, 119 (2) 494-499
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:95389
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:10 May 2019 18:09
Last Modified:03 Oct 2019 21:13

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