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Mapping of a mutation, polB100, affecting deoxyribonucleic acid polymerase II in Escherichia coli K-12

Campbell, Judith L. and Shizuya, Hiroaki and Richardson, Charles C. (1974) Mapping of a mutation, polB100, affecting deoxyribonucleic acid polymerase II in Escherichia coli K-12. Journal of Bacteriology, 119 (2). pp. 494-499. ISSN 0021-9193. PMCID PMC245632.

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Direct assay for deoxyribonucleic acid polymerase II in extracts has been used to screen recombinants for the polB allele in Hfr x F^- crosses, F-ductants in episome transfer, and transductants in generalized transduction by phage P1. The polB gene is located at 2 min on the Escherichia coli linkage map; it is 39 to 64% co-transducible with leu. A mutant, E. coli polA1 polB100 polC (ts), deficient in deoxyribonucleic acid polymerases I and II and having a thermolabile deoxyribonucleic acid polymerase III, has been prepared by the P1-mediated cross: P1 (HMS85 polB100) x E. coli BT1026 polA1 polC (ts) leu^-.

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Additional Information:© 1974 American Society for Microbiology. Received for publication 26 April 1974. This investigation was supported by Public Health Service grant AI-06045 from the National Institute of Allergy and Infectious Diseases, grant NP-1C from the American Cancer Society, Incorporated, and Public Health Service Research Career Development Award GM 13,634 (to C.C.R.) from the National Institute of General Medical Sciences.
Funding AgencyGrant Number
American Cancer SocietyNP-1C
NIHGM 13634
Issue or Number:2
PubMed Central ID:PMC245632
Record Number:CaltechAUTHORS:20190510-105514765
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Official Citation:Mapping of a Mutation, polB100, Affecting Deoxyribonucleic Acid Polymerase II in Escherichia coli K-12 Judith L. Campbell, Hiroaki Shizuya, Charles C. Richardson Journal of Bacteriology Aug 1974, 119 (2) 494-499
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:95389
Deposited By: George Porter
Deposited On:10 May 2019 18:09
Last Modified:03 Oct 2019 21:13

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