Campbell, Judith L. and Shizuya, Hiroaki and Richardson, Charles C. (1974) Mapping of a mutation, polB100, affecting deoxyribonucleic acid polymerase II in Escherichia coli K-12. Journal of Bacteriology, 119 (2). pp. 494-499. ISSN 0021-9193. PMCID PMC245632. https://resolver.caltech.edu/CaltechAUTHORS:20190510-105514765
![[img]](https://authors.library.caltech.edu/style/images/fileicons/application_pdf.png) |
PDF
- Published Version
See Usage Policy. 842kB |
Use this Persistent URL to link to this item: https://resolver.caltech.edu/CaltechAUTHORS:20190510-105514765
Abstract
Direct assay for deoxyribonucleic acid polymerase II in extracts has been used to screen recombinants for the polB allele in Hfr x F^- crosses, F-ductants in episome transfer, and transductants in generalized transduction by phage P1. The polB gene is located at 2 min on the Escherichia coli linkage map; it is 39 to 64% co-transducible with leu. A mutant, E. coli polA1 polB100 polC (ts), deficient in deoxyribonucleic acid polymerases I and II and having a thermolabile deoxyribonucleic acid polymerase III, has been prepared by the P1-mediated cross: P1 (HMS85 polB100) x E. coli BT1026 polA1 polC (ts) leu^-.
| Item Type: | Article | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Related URLs: |
| |||||||||
| Additional Information: | © 1974 American Society for Microbiology. Received for publication 26 April 1974. This investigation was supported by Public Health Service grant AI-06045 from the National Institute of Allergy and Infectious Diseases, grant NP-1C from the American Cancer Society, Incorporated, and Public Health Service Research Career Development Award GM 13,634 (to C.C.R.) from the National Institute of General Medical Sciences. | |||||||||
| Funders: |
| |||||||||
| Issue or Number: | 2 | |||||||||
| PubMed Central ID: | PMC245632 | |||||||||
| Record Number: | CaltechAUTHORS:20190510-105514765 | |||||||||
| Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20190510-105514765 | |||||||||
| Official Citation: | Mapping of a Mutation, polB100, Affecting Deoxyribonucleic Acid Polymerase II in Escherichia coli K-12 Judith L. Campbell, Hiroaki Shizuya, Charles C. Richardson Journal of Bacteriology Aug 1974, 119 (2) 494-499 | |||||||||
| Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | |||||||||
| ID Code: | 95389 | |||||||||
| Collection: | CaltechAUTHORS | |||||||||
| Deposited By: | George Porter | |||||||||
| Deposited On: | 10 May 2019 18:09 | |||||||||
| Last Modified: | 03 Oct 2019 21:13 |
Repository Staff Only: item control page
